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Title: Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988

Abstract

Characterizing the polysaccharides that constitute the primary cell walls of plants has been a major research goal of our laboratory for many years. Our experience and the vastly improved technologies used to study these polysaccharides have resulted in a steadily increasing rate at which we are acquiring structural knowledge of the cell wall. We have recently completed projects that have identified the major structural features of the pectic polysaccharide rhamnogalacturonan I in monocots and in describing the rhamnogalacturonan I family found in dicot tissues. We have also made considerable progress toward elucidating the structure of the pectic polysaccharide rhamnogalacturonan II isolated from sycamore cell walls and completed the identification of 3-deoxy-D-lyxo-2-heptulosaric acids, a component of rhamnogalacturonan II. We have also recently completed studies on the comparison of the O-acetylation pattern of xyloglucans isolated from sycamore cell walls and xyloglucans isolated from the extracellular medium of sycamore cells grown in culture. Finally, we have completed work comparing various methods of preparing cell walls in order to optimize the yield of cell wall fractions solubilized by chemical extraction procedures. However, in spite of this substantial progress, we remain far from defining the complete primary structures of the noncellulosic polysaccharides of cell walls.more » 8 figs.« less

Authors:
Publication Date:
Research Org.:
Georgia Univ., Athens (USA). Complex Carbohydrate Research Center
OSTI Identifier:
6518233
Report Number(s):
DOE/ER/13426-2
ON: DE89005911
DOE Contract Number:  
FG09-85ER13426
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL WALL; CHEMICAL COMPOSITION; CHEMICAL WASTES; FRACTIONATION; POLYSACCHARIDES; STRUCTURE-ACTIVITY RELATIONSHIPS; EVALUATION; PLANTS; PROGRESS REPORT; CARBOHYDRATES; CELL CONSTITUENTS; DOCUMENT TYPES; ORGANIC COMPOUNDS; SACCHARIDES; SEPARATION PROCESSES; WASTES; 550200* - Biochemistry

Citation Formats

Darvill, A. Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988. United States: N. p., 1988. Web.
Darvill, A. Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988. United States.
Darvill, A. Thu . "Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988". United States.
@article{osti_6518233,
title = {Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988},
author = {Darvill, A},
abstractNote = {Characterizing the polysaccharides that constitute the primary cell walls of plants has been a major research goal of our laboratory for many years. Our experience and the vastly improved technologies used to study these polysaccharides have resulted in a steadily increasing rate at which we are acquiring structural knowledge of the cell wall. We have recently completed projects that have identified the major structural features of the pectic polysaccharide rhamnogalacturonan I in monocots and in describing the rhamnogalacturonan I family found in dicot tissues. We have also made considerable progress toward elucidating the structure of the pectic polysaccharide rhamnogalacturonan II isolated from sycamore cell walls and completed the identification of 3-deoxy-D-lyxo-2-heptulosaric acids, a component of rhamnogalacturonan II. We have also recently completed studies on the comparison of the O-acetylation pattern of xyloglucans isolated from sycamore cell walls and xyloglucans isolated from the extracellular medium of sycamore cells grown in culture. Finally, we have completed work comparing various methods of preparing cell walls in order to optimize the yield of cell wall fractions solubilized by chemical extraction procedures. However, in spite of this substantial progress, we remain far from defining the complete primary structures of the noncellulosic polysaccharides of cell walls. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {9}
}

Technical Report:
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