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Title: Interaction of oxygen concentration, radiation dose, and seed water content on the oxygen effect in irradiated barley seeds. [. gamma. rays]

Abstract

The roles of oxygen concentration, seed water content, and their interaction with concentration of radiation-induced oxygen-sensitive sites in the initiation of damage to dry barley seeds were investigated. Seeds irradiated in evacuated glass vials with /sup 60/Co were hydrated at 0/sup 0/C in distilled water bubbled with oxygen--nitrogen gas mixtures containing 0.0, 3.1, 6.25, 12.5, 25, 50, and 100% oxygen. Biological effects of the treatments were recorded as M/sub 1/ seedling injury. Practically no oxygen effect was obtained with an oxygen concentration of 3.1% in the hydration gas (OCHG). The minimum OCHG needed to show an O/sub 2/ effect increased with increasing seed water content between 1.8 and 10.0%, and decreased as the radiation dose increased, suggesting a triple factor interaction. For moderate levels of injury, a nearly linear increase in seedling injury was obtained when the OCHG was increased in an exponential fashion. For greater seedling injury, the response tended toward a sigmoid shaped curve. The same types of response were obtained when the results were analyzed in terms of oxygen enhancement ratios (OER) obtained from seedling injury data. Decreases in oxygen independent damage were obtained as the seed water content was increased from 2.0 to 7.7%. A proportionatelymore » smaller increase in the dose was required between 7.7 and 9.9% seed water content. The same pattern of radiosensitivity was observed with aerobic hydrations and was more pronounced at intermediate levels of OCHG. The increase in response at intermediate OCHG was in part a reflection of the influence of seed water content on the level of OCHG needed to produce an oxygen enhancement of damage. Cooling seeds of 6.1% water content to dry ice temperatures immediately after irradiation at 0/sup 0/C decreased both oxygen dependent and oxygen independent damage. The decreases in damage were greater at 20 krads than at 10 krads and tended to be greater at the higher OCHG.« less

Authors:
; ;
Publication Date:
Research Org.:
Washington State Univ., Pullman (USA). Dept. of Agronomy and Soils
OSTI Identifier:
6509374
Report Number(s):
RLO-2221-T2-39
TRN: 79-005787
DOE Contract Number:  
EY-76-S-06-2221-002
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LOW TEMPERATURE; RADIOSENSITIVITY EFFECTS; MOISTURE; OXYGEN; RADIATION DOSES; SEEDS; BIOLOGICAL RADIATION EFFECTS; RADIOSENSITIVITY; BARLEY; COBALT 60; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; IRRADIATION; OXYGEN ENHANCEMENT RATIO; RESPONSE MODIFYING FACTORS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CEREALS; COBALT ISOTOPES; CRYOGENIC FLUIDS; DOSES; ELECTROMAGNETIC RADIATION; ELEMENTS; FLUIDS; GRAMINEAE; GRASS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NONMETALS; NUCLEI; ODD-ODD NUCLEI; PLANTS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES; 560141* - Radiation Effects on Plants- Basic Studies- (-1987)

Citation Formats

Donaldson, E, Nilan, R A, and Konzak, C F. Interaction of oxygen concentration, radiation dose, and seed water content on the oxygen effect in irradiated barley seeds. [. gamma. rays]. United States: N. p., 1978. Web.
Donaldson, E, Nilan, R A, & Konzak, C F. Interaction of oxygen concentration, radiation dose, and seed water content on the oxygen effect in irradiated barley seeds. [. gamma. rays]. United States.
Donaldson, E, Nilan, R A, and Konzak, C F. Sun . "Interaction of oxygen concentration, radiation dose, and seed water content on the oxygen effect in irradiated barley seeds. [. gamma. rays]". United States.
@article{osti_6509374,
title = {Interaction of oxygen concentration, radiation dose, and seed water content on the oxygen effect in irradiated barley seeds. [. gamma. rays]},
author = {Donaldson, E and Nilan, R A and Konzak, C F},
abstractNote = {The roles of oxygen concentration, seed water content, and their interaction with concentration of radiation-induced oxygen-sensitive sites in the initiation of damage to dry barley seeds were investigated. Seeds irradiated in evacuated glass vials with /sup 60/Co were hydrated at 0/sup 0/C in distilled water bubbled with oxygen--nitrogen gas mixtures containing 0.0, 3.1, 6.25, 12.5, 25, 50, and 100% oxygen. Biological effects of the treatments were recorded as M/sub 1/ seedling injury. Practically no oxygen effect was obtained with an oxygen concentration of 3.1% in the hydration gas (OCHG). The minimum OCHG needed to show an O/sub 2/ effect increased with increasing seed water content between 1.8 and 10.0%, and decreased as the radiation dose increased, suggesting a triple factor interaction. For moderate levels of injury, a nearly linear increase in seedling injury was obtained when the OCHG was increased in an exponential fashion. For greater seedling injury, the response tended toward a sigmoid shaped curve. The same types of response were obtained when the results were analyzed in terms of oxygen enhancement ratios (OER) obtained from seedling injury data. Decreases in oxygen independent damage were obtained as the seed water content was increased from 2.0 to 7.7%. A proportionately smaller increase in the dose was required between 7.7 and 9.9% seed water content. The same pattern of radiosensitivity was observed with aerobic hydrations and was more pronounced at intermediate levels of OCHG. The increase in response at intermediate OCHG was in part a reflection of the influence of seed water content on the level of OCHG needed to produce an oxygen enhancement of damage. Cooling seeds of 6.1% water content to dry ice temperatures immediately after irradiation at 0/sup 0/C decreased both oxygen dependent and oxygen independent damage. The decreases in damage were greater at 20 krads than at 10 krads and tended to be greater at the higher OCHG.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1978},
month = {1}
}

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