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Title: Effect of lung damage by acute exposure to nitrogen dioxide on lung immunity in the rat

Abstract

Exposure to nitrogen dioxide damages Type I alveolar epithelial cells and the epithelium of the terminal bronchiole. Because an intact epithelium may help control the number of inhaled particles that are cleared by the pulmonary lymphatics, damage to the alveolar epithelium could alter the antigen load to the lung-associated lymph nodes. To determine the effects of lung damage by NO/sub 2/ inhalation on lung immunity, we exposed adult, male rats to 26 ppM NO/sub 2/ for 24 h at various time intervals before and after intratracheal immunization with 10/sup 8/ sheep red blood cells. Seven days after immunization, we determined the number of anti-SRBC antibody-forming cells in the LALN, cervical lymph nodes, and spleen. The immunologic response to SRBC was limited to the LALN, with few or no AFC in either the cervical lymph nodes or spleen. A fivefold increase in the number of IgG anti-SRBC AFC/10/sup 6/ LALN cells was evident in rats immunized 1 day after NO/sub 2/ exposure. The increase was followed by a slight suppression of the IgG response when rats were immunized 3 days after exposure, returning to normal levels by 7 days after exposure. Histopathological examination of lung tissues showed a slight respiratory bronchiolitismore » which was followed by a bronchiolar epithelial cell hyperplasia and Type II cell hyperplasia in the adjacent alveoli. Based on these observations, it appears that the fluctuations observedin the LALN response may be the result of damage and subsequent repair of bronchiolar and alveolar epithelium following NO/sub 2/ inhalation.« less

Authors:
 [1]; ; ; ;
  1. Lovelace Inhalation Toxicology Research Inst., Albuquerque, NM
Publication Date:
OSTI Identifier:
6500183
DOE Contract Number:  
AC04-76EV01013
Resource Type:
Journal Article
Journal Name:
Environ. Res.; (United States)
Additional Journal Information:
Journal Volume: 23:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LUNGS; SENSITIVITY; NITROGEN DIOXIDE; BIOLOGICAL EFFECTS; TOXICITY; BRONCHI; EPITHELIUM; IMMUNITY; INHALATION; INHIBITION; LYMPHATIC SYSTEM; RATS; TIME DEPENDENCE; ANIMAL TISSUES; ANIMALS; BODY; CHALCOGENIDES; INTAKE; MAMMALS; NITROGEN COMPOUNDS; NITROGEN OXIDES; ORGANS; OXIDES; OXYGEN COMPOUNDS; RESPIRATORY SYSTEM; RODENTS; TISSUES; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Schnizlein, C T, Bice, D E, Rebar, A H, Wolff, R K, and Beethe, R L. Effect of lung damage by acute exposure to nitrogen dioxide on lung immunity in the rat. United States: N. p., 1980. Web. doi:10.1016/0013-9351(80)90071-7.
Schnizlein, C T, Bice, D E, Rebar, A H, Wolff, R K, & Beethe, R L. Effect of lung damage by acute exposure to nitrogen dioxide on lung immunity in the rat. United States. https://doi.org/10.1016/0013-9351(80)90071-7
Schnizlein, C T, Bice, D E, Rebar, A H, Wolff, R K, and Beethe, R L. Mon . "Effect of lung damage by acute exposure to nitrogen dioxide on lung immunity in the rat". United States. https://doi.org/10.1016/0013-9351(80)90071-7.
@article{osti_6500183,
title = {Effect of lung damage by acute exposure to nitrogen dioxide on lung immunity in the rat},
author = {Schnizlein, C T and Bice, D E and Rebar, A H and Wolff, R K and Beethe, R L},
abstractNote = {Exposure to nitrogen dioxide damages Type I alveolar epithelial cells and the epithelium of the terminal bronchiole. Because an intact epithelium may help control the number of inhaled particles that are cleared by the pulmonary lymphatics, damage to the alveolar epithelium could alter the antigen load to the lung-associated lymph nodes. To determine the effects of lung damage by NO/sub 2/ inhalation on lung immunity, we exposed adult, male rats to 26 ppM NO/sub 2/ for 24 h at various time intervals before and after intratracheal immunization with 10/sup 8/ sheep red blood cells. Seven days after immunization, we determined the number of anti-SRBC antibody-forming cells in the LALN, cervical lymph nodes, and spleen. The immunologic response to SRBC was limited to the LALN, with few or no AFC in either the cervical lymph nodes or spleen. A fivefold increase in the number of IgG anti-SRBC AFC/10/sup 6/ LALN cells was evident in rats immunized 1 day after NO/sub 2/ exposure. The increase was followed by a slight suppression of the IgG response when rats were immunized 3 days after exposure, returning to normal levels by 7 days after exposure. Histopathological examination of lung tissues showed a slight respiratory bronchiolitis which was followed by a bronchiolar epithelial cell hyperplasia and Type II cell hyperplasia in the adjacent alveoli. Based on these observations, it appears that the fluctuations observedin the LALN response may be the result of damage and subsequent repair of bronchiolar and alveolar epithelium following NO/sub 2/ inhalation.},
doi = {10.1016/0013-9351(80)90071-7},
url = {https://www.osti.gov/biblio/6500183}, journal = {Environ. Res.; (United States)},
number = ,
volume = 23:2,
place = {United States},
year = {1980},
month = {12}
}