Histidine-rich glycoprotein does not interfere with interactions between antithrombin III and heparin-like compounds on vascular endothelial cells

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Blood; (United States)

OSTI ID:6500069

Shimada, K; Kawamoto, A; Matsubayashi, K; Ozawa, T

The role of histidine-rich glycoprotein in controlling heparin-like compounds on the endothelial cell surface is still unclear. The effects of this heparin-neutralizing protein on the interaction between antithrombin III and cultured porcine aortic endothelial cells were examined. Displacement of /sup 125/I-labeled antithrombin III specifically bound to endothelial cells by unlabeled histidine-rich glycoprotein was much less potent than that by unlabeled antithrombin III. One hundred-fold molar excess of histidine-rich glycoprotein displaced specific /sup 125/I-antithrombin III binding only by 20%. Furthermore, the endothelial cell-mediated acceleration of thrombin inactivation by antithrombin III was diminished by protamine sulfate, but was not affected by histidine-rich glycoprotein even at a histidine-rich glycoprotein/antithrombin III molar ratio of approximately 7:1. These data indicate that histidine-rich glycoprotein does not interfere with the interaction of endothelial cell heparin-like compounds with antithrombin III. Thus, it may not play an important role in the modulation of anticoagulant activity of endothelial cells in vivo, suggesting that the commonly accepted view of the probable function of this protein is erroneous.

• Research Organization: Kochi Medical School (Japan)
• OSTI ID: 6500069
• Journal Information: Blood; (United States), Vol. 73:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
BIOLOGICAL FUNCTIONS
PROTEINS
BIOCHEMICAL REACTION KINETICS
AORTA
ENDOTHELIUM
HEPARIN
IODINE 125
RECEPTORS
SWINE
TRACER TECHNIQUES
AMINES
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYSACCHARIDES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques