Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study
The 577-nm flashlamp-pumped tunable dye laser pulsed at 450 microseconds is rapidly becoming the treatment of choice for removal of portwine stains and other vascular ectasias. In this study, we examined the mechanisms of vessel destruction by determining the effects of laser irradiation on three types of primary target cells--erythrocytes, endothelial cells, and fibroblasts. Human endothelial cells and fibroblasts in microwell plates were irradiated at various energy densities with the laser, after which several aspects of cellular biology were determined, including 1) viability of cells by trypan blue exclusion test; 2) cell proliferation by (3H)thymidine incorporation; and 3) rate of protein synthesis using (3H)leucine incorporation as a marker. In endothelial cell cultures, both (3H)thymidine and (3H)leucine incorporations were inhibited at energy levels of 5-12 J/cm2 (P less than 0.01). In fibroblast cultures, cell proliferation was similarly inhibited, while supratherapeutic energy density (greater than or equal to 12 J/cm2) was required for inhibition of protein synthesis. The laser energy in the range of 5-8.5 J/cm2 had no effect on cell viability. Erythrocytes as target cells for laser energy demonstrated rapid, dose-dependent lysis, as determined by release of free hemoglobin into culture medium. Addition of erythrocytes into a coculture with endothelial cells abolished the direct inhibitory effect noted in cultures when endothelial cells were present alone. The results of the latter experiment imply that erythrocytes are the primary target cell absorbing the laser energy at 577 nm. However, direct laser effects on endothelial cells may also contribute to the mechanisms of ablation of the vascular ectasias by the tunable dye laser at 577 nm.
- Research Organization:
- Jefferson Medical College, Philadelphia, PA (USA)
- OSTI ID:
- 6496102
- Journal Information:
- Lasers Surg. Med.; (United States), Vol. 8:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ERYTHROCYTES
CELL PROLIFERATION
FIBROBLASTS
LASER RADIATION
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
DYE LASERS
ENDOTHELIUM
IN VITRO
LEUCINE
LYSIS
SURGERY
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIABILITY
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LASERS
LIQUID LASERS
MATERIALS
MEDICINE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RIBOSIDES
SOMATIC CELLS
TISSUES
560400* - Other Environmental Pollutant Effects
550901 - Pathology- Tracer Techniques
550600 - Medicine