Role of exonucleolytic processing and polymerase-DNA association in bypass of lesions during replication in vitro. Significance for SOS-targeted mutagenesis
The role of exonuclease activity in trans-lesion DNA replication with Escherichia coli DNA polymerase III holoenzyme was investigated. RecA protein inhibited the 3'----5' exonuclease activity of the polymerase 2-fold when assayed in the absence of replication and had no effect on turnover of dNTPs into dNMPs. In contrast, single-stranded DNA-binding protein, which had no effect on the exonuclease activity in the absence of replication, showed a pronounced 7-fold suppression of the 3'----5' exonuclease activity during replication. The excision of incorporated dNMP alpha S residues from DNA by the 3'----5' exonuclease activity of DNA polymerase III holoenzyme was inhibited 10-20-fold; still no increase in bypass of pyrimidine photodimers was observed. Thus, in agreement with our previous results in which the exonuclease activity was inhibited at the protein level, inhibition at the DNA level also did not increase bypass of photodimers. Fractionation of the replication mixture after termination of DNA synthesis on a Bio-Gel A-5m column under conditions which favor polymerase-DNA binding yielded a termination complex which could perform turnover of dNTPs into dNMPs. Adding challenge-primed single-stranded DNA to the complex yielded a burst of DNA synthesis which was promoted most likely by DNA polymerase III holoenzyme molecules transferred from the termination complex to the challenge DNA thus demonstrating the instability of the polymerase-DNA association. Addition of a fresh sample of DNA polymerase III holoenzyme to purified termination products, which consist primarily of partially replicated molecules with nascent chains terminated at UV lesions, did not result in any net DNA synthesis as expected.
- Research Organization:
- Weizmann Inst. of Science, Rehovot (Israel)
- OSTI ID:
- 6485959
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:34
- Country of Publication:
- United States
- Language:
- English
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DNA POLYMERASES
BIOLOGICAL FUNCTIONS
DNA REPAIR
BIOCHEMICAL REACTION KINETICS
DNA REPLICATION
ENZYME ACTIVITY
ESCHERICHIA COLI
IN VITRO
MUTAGENESIS
PYRIMIDINE DIMERS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
FUNCTIONS
KINETICS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
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RECOVERY
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TRANSFERASES
560120* - Radiation Effects on Biochemicals
Cells
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