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Title: Modulation of thyroid hormone receptors by non-thyroidal stimuli

Abstract

The ability of non-thyroidal stimuli to affect the binding affinity and capacity of solubilized nuclear receptors for thyroid hormones was studied in a normal homeostatic system (erythropoiesis) and a pathobiologic one (lung-ozone interaction). No significant effects on affinity were found, as Kd control values for receptors derived from rat bone marrow averaged 57 (+/- 28) pM while experimental (hypoxic) values averaged 89 (+/- 55) pM. Kd control values in rat lung were found to average 142 (+/- 22) pM while average values derived from experimental protocols with ozone and methimazole were 267 (+/- 44) pM and 161 (+/- 35) pM respectively. Finally, Kd control values for receptors derived from cultured MEL cells averaged 19 (+/- 2.6) pM while experimental values during exposure to DMSO or IGF1 were 23 (+/- 3.6) pM and 26 (+/- 11) pM respectively. In contrast, binding capacity (expressed as fmoles of hormone bound per unit protein of solubilized receptor) was markedly perturbed in several tissues by various agents: ozone effects on lung were shown by an average control value of 3.3 (+/- 0.4) as opposed to an experimental average of 28 (+/- 1.9); and hypoxia effects on erythroid tissue were displayed by an average control valuemore » of 0.7 (+/- 0.07) as opposed to the experimental figure of 1.8 (+/- 0.03). In cultured MEL cells, binding capacity was seen to be increased from control values of 388 (+/- 15) sites/cell to 1243 (+/- 142) sites/cell after DMSO exposure and 2002 (+/- 10) sites/cell after IGF1 exposure. Parallel experiments done with receptors derived from rat liver yielded values similar to those reported by other investigators and were unaffected by the experimental agents.« less

Authors:
;
Publication Date:
Research Org.:
Central Univ. of Iowa, Department of Biology, Pella (USA)
OSTI Identifier:
6484846
Resource Type:
Journal Article
Resource Relation:
Journal Volume: 8:6
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DMSO; BIOLOGICAL EFFECTS; OZONE; THYROID HORMONES; RECEPTORS; ANOXIA; BIOCHEMICAL REACTION KINETICS; BONE MARROW; ERYTHROPOIETIN; LUNGS; RATS; STIMULI; ANIMAL TISSUES; ANIMALS; BODY; HEMATOPOIETIC SYSTEM; HORMONES; KINETICS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PEPTIDE HORMONES; PROTEINS; REACTION KINETICS; RESPIRATORY SYSTEM; RODENTS; SULFOXIDES; TISSUES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

ErkenBrack, D.E., and Clemons, G.K. Modulation of thyroid hormone receptors by non-thyroidal stimuli. United States: N. p., 1988. Web. doi:10.3109/10799898809049029.
ErkenBrack, D.E., & Clemons, G.K. Modulation of thyroid hormone receptors by non-thyroidal stimuli. United States. doi:10.3109/10799898809049029.
ErkenBrack, D.E., and Clemons, G.K. Fri . "Modulation of thyroid hormone receptors by non-thyroidal stimuli". United States. doi:10.3109/10799898809049029.
@article{osti_6484846,
title = {Modulation of thyroid hormone receptors by non-thyroidal stimuli},
author = {ErkenBrack, D.E. and Clemons, G.K.},
abstractNote = {The ability of non-thyroidal stimuli to affect the binding affinity and capacity of solubilized nuclear receptors for thyroid hormones was studied in a normal homeostatic system (erythropoiesis) and a pathobiologic one (lung-ozone interaction). No significant effects on affinity were found, as Kd control values for receptors derived from rat bone marrow averaged 57 (+/- 28) pM while experimental (hypoxic) values averaged 89 (+/- 55) pM. Kd control values in rat lung were found to average 142 (+/- 22) pM while average values derived from experimental protocols with ozone and methimazole were 267 (+/- 44) pM and 161 (+/- 35) pM respectively. Finally, Kd control values for receptors derived from cultured MEL cells averaged 19 (+/- 2.6) pM while experimental values during exposure to DMSO or IGF1 were 23 (+/- 3.6) pM and 26 (+/- 11) pM respectively. In contrast, binding capacity (expressed as fmoles of hormone bound per unit protein of solubilized receptor) was markedly perturbed in several tissues by various agents: ozone effects on lung were shown by an average control value of 3.3 (+/- 0.4) as opposed to an experimental average of 28 (+/- 1.9); and hypoxia effects on erythroid tissue were displayed by an average control value of 0.7 (+/- 0.07) as opposed to the experimental figure of 1.8 (+/- 0.03). In cultured MEL cells, binding capacity was seen to be increased from control values of 388 (+/- 15) sites/cell to 1243 (+/- 142) sites/cell after DMSO exposure and 2002 (+/- 10) sites/cell after IGF1 exposure. Parallel experiments done with receptors derived from rat liver yielded values similar to those reported by other investigators and were unaffected by the experimental agents.},
doi = {10.3109/10799898809049029},
journal = {},
number = ,
volume = 8:6,
place = {United States},
year = {1988},
month = {1}
}