Charge state reduction of oligonucleotide negative ions from electrospray ionization

Journal Article · Wed Feb 01 00:00:00 EST 1995 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00099a016· OSTI ID:6483873

Cheng, X; Gale, D C; Udseth, H R; Smith, R D ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States)

We have investigated the feasibility of simplifying the electrospray ionization (ESI) mass spectra for mixture analyses through charge state reduction. Two methods for charge state reduction of gas phase oligonucleotide negative ions were evaluated: (1) the addition of acids to the oligonucleotide solution and (2) the formation of diamine adducts followed by dissociation in the interface region. In the first method, the efficiency of charge state reduction depends on the pK[sub a], the concentration, and the nature of the acids. Acetic and formic acids were found to be better reagents than HCl, CF[sub 3]CO[sub 2]H, and H[sub 3]PO[sub 4]. The second method has the advantage that the stability of oligonucleotides is not affected but requires the optimization of the interface dissociation conditions and the amounts of diamine added to the oligonucleotide solution. Both methods show promise for charge state reduction, and results are presented for two oligonucleotides: d(pT)[sub 12] and d(AGCT). Substantial reduction in spectral complexity upon charge state reduction was also observed for a four-component mixture of oligonucleotides. 54 refs., 8 figs.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6483873
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 67:3; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CHARGE STATES
REDUCTION
IONS
MASS SPECTRA
OLIGONUCLEOTIDES
IONIZATION
ADDUCTS
CHEMICAL ANALYSIS
MIXTURES
ORGANIC ACIDS
CHARGED PARTICLES
CHEMICAL REACTIONS
DISPERSIONS
DNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SPECTRA
550200* - Biochemistry
400102 - Chemical & Spectral Procedures
400201 - Chemical & Physicochemical Properties