Mapping of the gene encoding the [beta]-subunit of H[sup +], K[sup +]-ATPase to human chromosome 13q34 by fluorescence in situ hybridization

Journal Article · Tue Dec 01 00:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80142-9· OSTI ID:6482974

Song, I; Brown, D R; Yamada, T; Trent, J M ^[1]

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1. Univ. of Michigan Medical Center, Ann Arbor, MI (United States)

To identify the chromosomal loci encoding this gene, 1 [mu]g of a clone of the human H[sup +], K[sup +]-ATPase subunit [beta] (designated HKB3-1-1a), which contains the coding region including exons 1 to 4 (approximately 17 kb), was labeled with biotin and hybridized to human metaphase chromosomes as previously described. A total of 32 metaphase cells were examined, and all cells examined had double fluorescent signals (i.e., one on each chromatid) on the terminal long arm of chromosome 13. In 17 cells double signals were observed on both homologs, and 15 cells had one homolog with a double signal on chromosome 13. Only chromosomes in which both chromatids displayed a signal were included for analyses, making the background hybridization essentially zero. The same cells hybridized for FISH had been previously G-banded (using trypsin Giemsa) and photographed to allow direct comparisons of the results. The results demonstrated that the sequences hybridizing to a DNA fragment that contains the coding region for the [beta]-subunit of H[sup +], K[sup +]-ATPase can be localized to 13q34. 16 refs., 1 fig.

• OSTI ID: 6482974
• Journal Information: Genomics; (United States), Vol. 14:4; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
GENES
HUMAN CHROMOSOME 13
GENETIC MAPPING
DNA HYBRIDIZATION
ACID ANHYDRASES
BIOTECHNOLOGY
CHROMOSOMES
ENZYMES
GENETIC ENGINEERING
HUMAN CHROMOSOMES
HYBRIDIZATION
HYDROLASES
MAPPING
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PROTEINS
550400* - Genetics