Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase

Journal Article · Tue Jun 01 00:00:00 EDT 1999 · Plant Physiology; (Bethesda)

DOI:https://doi.org/10.1104/pp.120.2.559· OSTI ID:6479955

Knaap, E van der; Sauter, M; Kende, H ^[1]; Song, W Y; Ruan, D L; Ronald, P C ^[2]

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1. Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.
2. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology

The authors identified in deepwater rice (Oryza sativa L.) a gene encoding a leucine-rich repeat receptor-like transmembrane protein kinase, OsTMK (O. sativa transmembrane kinase). The transcript levels of OsTMK increased in the rice internode in response to gibberellin. Expression of OsTMK was especially high in regions undergoing cell division and elongation. The kinase domain of OsTMK was enzymatically active autophosphorylating on serine and threonine residues. A cDNA encoding a rice ortholog of a kinase-associated type 2C protein phosphatase (OsKAPP) was cloned. KAPPs are putative downstream components in kinase-mediated signal transduction pathways. The kinase interaction domain of OsKAPP was phosphorylated in vitro by the kinase domain of OsTMK. RNA gel-blot analysis indicated that the expression of OsTMK and OsKAPP was similar in different tissues of the rice plant. In protein-binding assays, OsKAPP interacted with a receptor-like protein kinase, RLK5 of Arabidopsis, but not with the protein kinase domains of the rice and maize receptor-like protein kinases Xa21 and ZmPK1, respectively.

• Sponsoring Organization: National Science Foundation (NSF); USDOE; National Science Foundation, Washington, DC (United States); USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER20021
• OSTI ID: 6479955
• Journal Information: Plant Physiology; (Bethesda), Vol. 120:2; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
ENZYME ACTIVITY
GENE REGULATION
GENES
MEMBRANE PROTEINS
PHOSPHATASES
PHOSPHOTRANSFERASES
RICE
CEREALS
ENZYMES
ESTERASES
GRAMINEAE
HYDROLASES
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PROTEINS
REACTION KINETICS
TRANSFERASES
550200* - Biochemistry