Investigation of phospholipid synthesis and the disposition of amino acid and carbohydrate
The synthesis of pulmonary phospholipids by offspring of diabetic female rats was assessed by means of high performance liquid chromatography combined with automated phosphate analysis. No changes in the pool sizes of the major phospholipids or their precursors were observed. However, offspring of both insulin-treated and untreated diabetic mothers displayed increased pulmonary lyso-phosphatidylcholine. The concentration of glycerylphosphorylcholine, the metabolic product of lyso-phosphatidylcholine, was also increased in these offspring, providing further evidence of a reduced reacylation pathway in the offspring of diabetic mothers. The concentration of phosphatidylglycerol was reduced in the lungs from offspring of diabetic mothers. Preliminary investigation suggested that the mechanism of insulin action on lungs from offspring of diabetic rats may be the diversion of substrate from lipid synthetic pathways into protein synthesis. The utilization of (14C)-labeled amino acids and carbohydrates by normal fetal rat lung, however, revealed no direct insulin effect on protein synthesis. The ability of the fetal lung to convert amino acids into Krebs Cycle intermediates was demonstrated.
- OSTI ID:
- 6479138
- Country of Publication:
- United States
- Language:
- English
Similar Records
Changes in phospholipid biosynthetic enzyme activity in lung tissue and isolated lung cells of rats after exposure to nitrogen dioxide
Lactate metabolism in the fetal rabbit lung
Related Subjects
DIABETES MELLITUS
PATHOGENESIS
PHOSPHOLIPIDS
BIOSYNTHESIS
AMINO ACIDS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBON 14 COMPOUNDS
FETUSES
INSULIN
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
PROGENY
RATS
TRACER TECHNIQUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
ENDOCRINE DISEASES
ESTERS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
METABOLIC DISEASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques