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Title: Pulmonary metabolism of dibenz(a,j)acridine: A carcinogenic heterocyclic aromatic: Final report for period September 1, 1982-June 30, 1986

Abstract

The metabolism of the carcinogenic N-heterocyclic aromatic, dibenz(a,j)-acridine (DBA) was investigated in the isolated perfused lung (IPL) preparation. A significantly increased rate of metabolism was observed for DBA in benzo(a)-pyrene (BaP) and DBA-pretreated animals. This resulted in marked increases in conjugation, in particular sulfates and thioethers, and the distribution of conjugates and total metabolites in blood and lung. When Fe/sub 2/O/sub 3/ was coadministered with DBA to the IPL, the rate of metabolism was significantly decreased with respect to control experiments. This resulted in increased distributions of sulfate and thioether conjugates in blood. Spectroscopic analyses and microsomal enzyme analyses were used in the characterization of the four metabolites identified in the lung. The major nonconjugated metabolite was the 3,4 dihydrodiol of DBA and the three minor metabolites were the 4 and 3 phenol of DBA and a dihydroxy compound of DBA. The results indicate that in the lung DBA is metabolized in a manner similar to that of BaP but different from that of dibenzo-(c,g)carbazole. It is, therefore, apparent that the metabolism of N-heterocyclic aromatics are related to the aromaticity of the heteroatom-containing ring and the solubility of compound, as well as the specific enzymes responsible for activation of themore » compound. 115 refs., 78 figs., 24 tabs.« less

Authors:
Publication Date:
Research Org.:
Cincinnati Univ., OH (USA). Dept. of Environmental Health
OSTI Identifier:
6470799
Report Number(s):
DOE/ER/60076-3
ON: DE87012995
DOE Contract Number:  
AC02-82ER60076
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ACRIDINES; FLUORESCENCE SPECTROSCOPY; LIQUID COLUMN CHROMATOGRAPHY; MASS SPECTROSCOPY; METABOLISM; AZAARENES; METABOLITES; CARBON 14 COMPOUNDS; DOSE-RESPONSE RELATIONSHIPS; GLUCURONIDE CONJUGATES; INTRATRACHEAL ADMINISTRATION; INTRAVENOUS INJECTION; IRON OXIDES; LUNGS; METABOLIC ACTIVATION; PHENOLS; RABBITS; REVIEWS; SULFATES; TRACER TECHNIQUES; ANIMALS; AROMATICS; AZINES; BODY; CHALCOGENIDES; CHROMATOGRAPHY; DOCUMENT TYPES; EMISSION SPECTROSCOPY; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INJECTION; INTAKE; IRON COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; PYRIDINES; RESPIRATORY SYSTEM; SEPARATION PROCESSES; SPECTROSCOPY; SULFUR COMPOUNDS; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550501 - Metabolism- Tracer Techniques

Citation Formats

Warshawsky, D. Pulmonary metabolism of dibenz(a,j)acridine: A carcinogenic heterocyclic aromatic: Final report for period September 1, 1982-June 30, 1986. United States: N. p., 1987. Web.
Warshawsky, D. Pulmonary metabolism of dibenz(a,j)acridine: A carcinogenic heterocyclic aromatic: Final report for period September 1, 1982-June 30, 1986. United States.
Warshawsky, D. Wed . "Pulmonary metabolism of dibenz(a,j)acridine: A carcinogenic heterocyclic aromatic: Final report for period September 1, 1982-June 30, 1986". United States.
@article{osti_6470799,
title = {Pulmonary metabolism of dibenz(a,j)acridine: A carcinogenic heterocyclic aromatic: Final report for period September 1, 1982-June 30, 1986},
author = {Warshawsky, D},
abstractNote = {The metabolism of the carcinogenic N-heterocyclic aromatic, dibenz(a,j)-acridine (DBA) was investigated in the isolated perfused lung (IPL) preparation. A significantly increased rate of metabolism was observed for DBA in benzo(a)-pyrene (BaP) and DBA-pretreated animals. This resulted in marked increases in conjugation, in particular sulfates and thioethers, and the distribution of conjugates and total metabolites in blood and lung. When Fe/sub 2/O/sub 3/ was coadministered with DBA to the IPL, the rate of metabolism was significantly decreased with respect to control experiments. This resulted in increased distributions of sulfate and thioether conjugates in blood. Spectroscopic analyses and microsomal enzyme analyses were used in the characterization of the four metabolites identified in the lung. The major nonconjugated metabolite was the 3,4 dihydrodiol of DBA and the three minor metabolites were the 4 and 3 phenol of DBA and a dihydroxy compound of DBA. The results indicate that in the lung DBA is metabolized in a manner similar to that of BaP but different from that of dibenzo-(c,g)carbazole. It is, therefore, apparent that the metabolism of N-heterocyclic aromatics are related to the aromaticity of the heteroatom-containing ring and the solubility of compound, as well as the specific enzymes responsible for activation of the compound. 115 refs., 78 figs., 24 tabs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {7}
}

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