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Title: Development and characterization of a fiber optic immuno biosensor

Abstract

In this paper we describe preliminary results from the development and characterization of a fiber optic biosensor that utilizes a fluorescently-labeled monoclonal antibody to pyrethroid pesticides. A synthetic pyrethroid-like antigen was coupled to bovine serum albumin and immobilized on the surface of a stripped portion of the distal end of an optical fiber. Each fiber was individually calibrated for its optical response using standard solutions of fluoresceinamine. Binding of the antibody was detected via the evanescent wave of the fiber by use of a portable fiber optic fluorimeter. Initial binding rate and equilibrium levels of bound antibody showed a linear relationship with antibody concentrations between 7 nM and 25 uM. To test the reversibility of the biosensor, bound antibody was removed with protein denaturing agents and the same fibers were re-tested with similar concentrations of antibody. When re-immersed in antibody solution, binding of the antibodies was again observed. The results provide encouraging evidence that such a biosensor may be useful for the determination of small organic molecules in situ. 17 refs., 8 figs.

Authors:
; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab., CA (USA)
OSTI Identifier:
6468034
Report Number(s):
UCRL-100110; CONF-8811163-1
ON: DE89005062
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: NATO Advance Study Institute on spectroscopy of inorganic bioactivators, Loutraki, Greece, 1 Nov 1988; Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 47 OTHER INSTRUMENTATION; IMMUNOASSAY; DESIGN; PESTICIDES; DETECTION; EVALUATION; FIBER OPTICS; MEAT; MONITORING; MONOCLONAL ANTIBODIES; PRODUCTION; ANTIBODIES; FOOD; 550600* - Medicine; 440300 - Miscellaneous Instruments- (-1989)

Citation Formats

Northrup, M A, Stanker, L H, Vanderlaan, M, and Watkins, B E. Development and characterization of a fiber optic immuno biosensor. United States: N. p., 1988. Web.
Northrup, M A, Stanker, L H, Vanderlaan, M, & Watkins, B E. Development and characterization of a fiber optic immuno biosensor. United States.
Northrup, M A, Stanker, L H, Vanderlaan, M, and Watkins, B E. Fri . "Development and characterization of a fiber optic immuno biosensor". United States.
@article{osti_6468034,
title = {Development and characterization of a fiber optic immuno biosensor},
author = {Northrup, M A and Stanker, L H and Vanderlaan, M and Watkins, B E},
abstractNote = {In this paper we describe preliminary results from the development and characterization of a fiber optic biosensor that utilizes a fluorescently-labeled monoclonal antibody to pyrethroid pesticides. A synthetic pyrethroid-like antigen was coupled to bovine serum albumin and immobilized on the surface of a stripped portion of the distal end of an optical fiber. Each fiber was individually calibrated for its optical response using standard solutions of fluoresceinamine. Binding of the antibody was detected via the evanescent wave of the fiber by use of a portable fiber optic fluorimeter. Initial binding rate and equilibrium levels of bound antibody showed a linear relationship with antibody concentrations between 7 nM and 25 uM. To test the reversibility of the biosensor, bound antibody was removed with protein denaturing agents and the same fibers were re-tested with similar concentrations of antibody. When re-immersed in antibody solution, binding of the antibodies was again observed. The results provide encouraging evidence that such a biosensor may be useful for the determination of small organic molecules in situ. 17 refs., 8 figs.},
doi = {},
url = {https://www.osti.gov/biblio/6468034}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {12}
}

Conference:
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