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Title: Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes

Abstract

Plasma /sup 125/I-albumin was used as a marker of extracellular dilution in order to study the effect of high-speed centrifugation on transmembrane water distribution in several types of human red cells, including normal (AA), hemoglobin variants (beta A, AS, SC, beta S, and SS), and those from patients with hereditary spherocytosis. SS and AA erythrocytes were also examined for changes in intracellular hemoglobin concentration of three different density fractions and with increasing duration of spin. The minimum force and duration of centrifugation required to impair water permeability were found to vary with the red cell type, the anticoagulant used (heparin or EDTA), the initial hematocrit of the sample centrifuged, as well as among the individual erythrocyte fractions within the same sample. When subjecting pathologic erythrocytes to high-speed centrifugation, the /sup 125/I-albumin dilution technique can be used to determine whether the centrifugation procedure has led to an artifactual red cell water loss and to correct for this when it does occur. An abnormal membrane susceptibility to mechanical stress was demonstrated in erythrocytes from patients with hereditary spherocytosis and several hemoglobinopathies.

Authors:
;
Publication Date:
Research Org.:
Columbia Univ., New York, NY (USA)
OSTI Identifier:
6462997
Resource Type:
Journal Article
Journal Name:
Proc. Soc. Exp. Biol. Med.; (United States)
Additional Journal Information:
Journal Volume: 190:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; PERMEABILITY; CENTRIFUGATION; BIOLOGICAL EFFECTS; HEMIC DISEASES; PATHOGENESIS; WATER; MEMBRANE TRANSPORT; ALBUMINS; BLOOD COUNT; ERYTHROCYTES; IODINE 125; ISOTOPE DILUTION; MAN; THALASSEMIA; ANEMIAS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HYDROGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PRIMATES; PROTEINS; RADIOISOTOPES; SEPARATION PROCESSES; SYMPTOMS; TRACER TECHNIQUES; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Kaperonis, A A, and Chien, S. Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes. United States: N. p., 1989. Web. doi:10.3181/00379727-190-42846.
Kaperonis, A A, & Chien, S. Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes. United States. https://doi.org/10.3181/00379727-190-42846
Kaperonis, A A, and Chien, S. 1989. "Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes". United States. https://doi.org/10.3181/00379727-190-42846.
@article{osti_6462997,
title = {Effects of centrifugation on transmembrane water loss from normal and pathologic erythrocytes},
author = {Kaperonis, A A and Chien, S},
abstractNote = {Plasma /sup 125/I-albumin was used as a marker of extracellular dilution in order to study the effect of high-speed centrifugation on transmembrane water distribution in several types of human red cells, including normal (AA), hemoglobin variants (beta A, AS, SC, beta S, and SS), and those from patients with hereditary spherocytosis. SS and AA erythrocytes were also examined for changes in intracellular hemoglobin concentration of three different density fractions and with increasing duration of spin. The minimum force and duration of centrifugation required to impair water permeability were found to vary with the red cell type, the anticoagulant used (heparin or EDTA), the initial hematocrit of the sample centrifuged, as well as among the individual erythrocyte fractions within the same sample. When subjecting pathologic erythrocytes to high-speed centrifugation, the /sup 125/I-albumin dilution technique can be used to determine whether the centrifugation procedure has led to an artifactual red cell water loss and to correct for this when it does occur. An abnormal membrane susceptibility to mechanical stress was demonstrated in erythrocytes from patients with hereditary spherocytosis and several hemoglobinopathies.},
doi = {10.3181/00379727-190-42846},
url = {https://www.osti.gov/biblio/6462997}, journal = {Proc. Soc. Exp. Biol. Med.; (United States)},
number = ,
volume = 190:2,
place = {United States},
year = {Wed Feb 01 00:00:00 EST 1989},
month = {Wed Feb 01 00:00:00 EST 1989}
}