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Title: Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body

Abstract

The intestinal absorption of an oral dose of zinc chloride was determined from the ratio of /sup 65/Zn and a nonabsorbed radioactive marker, /sup 51/Cr, present in a single stool specimen or the body 24-72 h later. Chromic chloride had no effect on (/sup 65/Zn)zinc chloride absorption and /sup 51/Cr and /sup 65/Zn had similar intestinal transit times. In 17 healthy control subtects given 92 mumol ZnCl/sub 2/ labeled with 0.5 microCi /sup 65/Zn, 52 +/- 14% (SD) of the dose was taken up from the lumen. Intestinal absorption of /sup 65/Zn at 24 h correlated closely with /sup 65/Zn body retention of zinc measured by whole-body counting 7 days later, r . 0.995. Neither zinc absorption nor zinc retention correlated with blood leukocyte zinc levels. An average of 55% of /sup 65/Zn was retained in the body from doses of 18-90 mumol ZnCl/sub 2/ but a progressively smaller proportion of zinc was absorbed from doses of 180-900 mumol. The average absorption and body retention of /sup 65/Zn were significantly reduced in 7 patients with mucosal disease of the proximal intestine but they were not affected by resection of the lower jejunum, ileum, and colon. Thus the absorption of ZnCl/submore » 2/ from a 92-mumol dose predominantly takes place by a rate-limited mechanism in the duodenum and upper jejunum.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Departments of Medicine and Nuclear Medicine, University Hospital, London, Ontario, Canada
OSTI Identifier:
6456337
Resource Type:
Journal Article
Journal Name:
Gastroenterology; (United States)
Additional Journal Information:
Journal Volume: 83:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMIUM 51; ISOTOPE APPLICATIONS; ZINC; INTESTINAL ABSORPTION; ZINC 65; DIGESTIVE SYSTEM DISEASES; FECES; LEUKOCYTES; MUCOUS MEMBRANES; PATHOLOGY; PATIENTS; RETENTION; ZINC CHLORIDES; ABSORPTION; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BLOOD; BLOOD CELLS; BODY FLUIDS; CHLORIDES; CHLORINE COMPOUNDS; CHROMIUM ISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; HALIDES; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; MATERIALS; MEMBRANES; METALS; NUCLEI; RADIOISOTOPES; UPTAKE; WASTES; ZINC COMPOUNDS; ZINC HALIDES; ZINC ISOTOPES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques

Citation Formats

Payton, K B, Flanagan, P R, Stinson, E A, Chodirker, D P, Chamberlain, M J, and Valberg, L S. Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body. United States: N. p., 1982. Web.
Payton, K B, Flanagan, P R, Stinson, E A, Chodirker, D P, Chamberlain, M J, & Valberg, L S. Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body. United States.
Payton, K B, Flanagan, P R, Stinson, E A, Chodirker, D P, Chamberlain, M J, and Valberg, L S. Wed . "Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body". United States.
@article{osti_6456337,
title = {Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body},
author = {Payton, K B and Flanagan, P R and Stinson, E A and Chodirker, D P and Chamberlain, M J and Valberg, L S},
abstractNote = {The intestinal absorption of an oral dose of zinc chloride was determined from the ratio of /sup 65/Zn and a nonabsorbed radioactive marker, /sup 51/Cr, present in a single stool specimen or the body 24-72 h later. Chromic chloride had no effect on (/sup 65/Zn)zinc chloride absorption and /sup 51/Cr and /sup 65/Zn had similar intestinal transit times. In 17 healthy control subtects given 92 mumol ZnCl/sub 2/ labeled with 0.5 microCi /sup 65/Zn, 52 +/- 14% (SD) of the dose was taken up from the lumen. Intestinal absorption of /sup 65/Zn at 24 h correlated closely with /sup 65/Zn body retention of zinc measured by whole-body counting 7 days later, r . 0.995. Neither zinc absorption nor zinc retention correlated with blood leukocyte zinc levels. An average of 55% of /sup 65/Zn was retained in the body from doses of 18-90 mumol ZnCl/sub 2/ but a progressively smaller proportion of zinc was absorbed from doses of 180-900 mumol. The average absorption and body retention of /sup 65/Zn were significantly reduced in 7 patients with mucosal disease of the proximal intestine but they were not affected by resection of the lower jejunum, ileum, and colon. Thus the absorption of ZnCl/sub 2/ from a 92-mumol dose predominantly takes place by a rate-limited mechanism in the duodenum and upper jejunum.},
doi = {},
journal = {Gastroenterology; (United States)},
number = ,
volume = 83:6,
place = {United States},
year = {1982},
month = {12}
}