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Title: Immunochemical characterization of the plasmin-antiplasmin system. Basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. [/sup 125/I]

Abstract

The capacity to discriminate the PAP from its constituents provides an index of activation of the fibrinolytic system. Rabbit antisera to human PAP, when optimally absorbed with plasminogen and AP and coupled to latex particles, effectively discriminated PAP from its precursors. A 100- to 500-fold excess of the precursors of PAP failed to produce particle agglutination. In primary binding radioimmunoassays, however, the antigen-binding capacities of the absorbed antisera were only 1.3 to 3.5 times higher for PAP than for AP. In competitive-inhibition radioimmunoassays, both produced complete inhibition, indicating the absence of absolute antigenic differences, and PAP was only a twofold to fourfold better competitor than AP. Differences in the discriminatory capacities of the latex and radioimmunoassay systems were not due to pH or ionic strength and could not be attributed to the radioiodination procedure. /sup 125/I-PAP was bound to the antibody-coated latex particles at a much faster rate than AP, but ultimately both were bound to an equal extent. Thus discrimination in the latex system was due to the more rapid binding of PAP. In an independent system, antibodies raised to plasminogen effectively discriminated plasminogen from PAP in a latex system but not in an equilibrium competitive-inhibition radioimmunoassay, suggesting thatmore » this concept was generally applicable. Differences in relative association rates appear to be maximized by rapid analysis, and this phenomenon may be of general utility for the discrimination of reaction products from their precursors by particle agglutination assays.« less

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Leuven, Belgium
OSTI Identifier:
6453873
Alternate Identifier(s):
OSTI ID: 6453873
Resource Type:
Journal Article
Journal Name:
J. Lab. Clin. Med.; (United States)
Additional Journal Information:
Journal Volume: 93:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; RADIOIMMUNOASSAY; EXPERIMENTAL DATA; GRAPHS; IMMUNE REACTIONS; IMMUNE SERUMS; IODINE 125; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; DATA; DATA FORMS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INFORMATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; RADIOASSAY; RADIOISOTOPES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics

Citation Formats

Plow, E.F., Cock, F.D., and Collen, D. Immunochemical characterization of the plasmin-antiplasmin system. Basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. [/sup 125/I]. United States: N. p., 1979. Web.
Plow, E.F., Cock, F.D., & Collen, D. Immunochemical characterization of the plasmin-antiplasmin system. Basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. [/sup 125/I]. United States.
Plow, E.F., Cock, F.D., and Collen, D. Thu . "Immunochemical characterization of the plasmin-antiplasmin system. Basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. [/sup 125/I]". United States.
@article{osti_6453873,
title = {Immunochemical characterization of the plasmin-antiplasmin system. Basis for the specific detection of the plasmin-antiplasmin complex by latex agglutination assays. [/sup 125/I]},
author = {Plow, E.F. and Cock, F.D. and Collen, D.},
abstractNote = {The capacity to discriminate the PAP from its constituents provides an index of activation of the fibrinolytic system. Rabbit antisera to human PAP, when optimally absorbed with plasminogen and AP and coupled to latex particles, effectively discriminated PAP from its precursors. A 100- to 500-fold excess of the precursors of PAP failed to produce particle agglutination. In primary binding radioimmunoassays, however, the antigen-binding capacities of the absorbed antisera were only 1.3 to 3.5 times higher for PAP than for AP. In competitive-inhibition radioimmunoassays, both produced complete inhibition, indicating the absence of absolute antigenic differences, and PAP was only a twofold to fourfold better competitor than AP. Differences in the discriminatory capacities of the latex and radioimmunoassay systems were not due to pH or ionic strength and could not be attributed to the radioiodination procedure. /sup 125/I-PAP was bound to the antibody-coated latex particles at a much faster rate than AP, but ultimately both were bound to an equal extent. Thus discrimination in the latex system was due to the more rapid binding of PAP. In an independent system, antibodies raised to plasminogen effectively discriminated plasminogen from PAP in a latex system but not in an equilibrium competitive-inhibition radioimmunoassay, suggesting that this concept was generally applicable. Differences in relative association rates appear to be maximized by rapid analysis, and this phenomenon may be of general utility for the discrimination of reaction products from their precursors by particle agglutination assays.},
doi = {},
journal = {J. Lab. Clin. Med.; (United States)},
number = ,
volume = 93:2,
place = {United States},
year = {1979},
month = {2}
}