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Title: Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone

Abstract

Three groups of 28 1-month-old male Sprague-Dawley rats each were fed a basal vitamin E-deficient diet and supplemented with either 0, 11, or 110 ppM vitamin E for 38 days, and were then exposed to 0 or 0.1 ppM ozone continuously for 7 days. Following ozone exposure, the level of reduced glutathione (GSH) and activities of GSH peroxidase, GSH reductase, glucose-6-phosphate dehydrogenase (G-6-PD), and lactate dehydrogenase (LDH), but not of malic dehydrogenase, were significantly elevated in the lungs of rats fed the vitamin E deficient diet. The level of GSH and activities of GSH peroxidase and G-6-PD were also significantly increased in the lungs of the animal group fed the 11 ppM vitamin E diet, while none of the biochemical measurements made was significantly altered by ozone in the 110-ppM vitamin E diet fed rats. Scanning electron microscope examination revealed that five out of six rats on the vitamin E-deficient diet and four out of six from the 11-ppM vitamin E diet had detectable lesions following ozone exposure, as compared with only one of the six exposed animals from the 110-ppM vitamin E diet. The lesion was restricted to bronchiolar epithelium and alveoli immediately adjacent to the bronchiole-alveolar duct junction.more » None of the control animals had detectable lesions. The results suggest that exposure to ozone at 0.1-ppM level can produce detectable pulmonary damage, and that dietary vitamin E alters pulmonary susceptibility to ozone exposure.« less

Authors:
 [1]; ; ;
  1. Univ. of Kentucky, Lexington
Publication Date:
OSTI Identifier:
6453422
Resource Type:
Journal Article
Journal Name:
Environ. Res.; (United States)
Additional Journal Information:
Journal Volume: 24:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; OZONE; TOXICITY; RESPIRATORY SYSTEM; SENSITIVITY; VITAMIN E; BIOLOGICAL EFFECTS; BIOCHEMISTRY; COMPARATIVE EVALUATIONS; DIET; ENZYME ACTIVITY; GLUTATHIONE; METABOLISM; MORPHOLOGICAL CHANGES; MORPHOLOGY; NUTRITIONAL DEFICIENCY; QUANTITY RATIO; RATS; TIME DEPENDENCE; ANIMALS; CHEMISTRY; DRUGS; MAMMALS; ORGANIC COMPOUNDS; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOPROTECTIVE SUBSTANCES; RODENTS; VERTEBRATES; VITAMINS; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 550200 - Biochemistry

Citation Formats

Chow, C K, Plopper, C G, Chiu, M, and Dungworth, D L. Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone. United States: N. p., 1981. Web. doi:10.1016/0013-9351(81)90161-4.
Chow, C K, Plopper, C G, Chiu, M, & Dungworth, D L. Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone. United States. doi:10.1016/0013-9351(81)90161-4.
Chow, C K, Plopper, C G, Chiu, M, and Dungworth, D L. Wed . "Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone". United States. doi:10.1016/0013-9351(81)90161-4.
@article{osti_6453422,
title = {Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0. 1 ppM ozone},
author = {Chow, C K and Plopper, C G and Chiu, M and Dungworth, D L},
abstractNote = {Three groups of 28 1-month-old male Sprague-Dawley rats each were fed a basal vitamin E-deficient diet and supplemented with either 0, 11, or 110 ppM vitamin E for 38 days, and were then exposed to 0 or 0.1 ppM ozone continuously for 7 days. Following ozone exposure, the level of reduced glutathione (GSH) and activities of GSH peroxidase, GSH reductase, glucose-6-phosphate dehydrogenase (G-6-PD), and lactate dehydrogenase (LDH), but not of malic dehydrogenase, were significantly elevated in the lungs of rats fed the vitamin E deficient diet. The level of GSH and activities of GSH peroxidase and G-6-PD were also significantly increased in the lungs of the animal group fed the 11 ppM vitamin E diet, while none of the biochemical measurements made was significantly altered by ozone in the 110-ppM vitamin E diet fed rats. Scanning electron microscope examination revealed that five out of six rats on the vitamin E-deficient diet and four out of six from the 11-ppM vitamin E diet had detectable lesions following ozone exposure, as compared with only one of the six exposed animals from the 110-ppM vitamin E diet. The lesion was restricted to bronchiolar epithelium and alveoli immediately adjacent to the bronchiole-alveolar duct junction. None of the control animals had detectable lesions. The results suggest that exposure to ozone at 0.1-ppM level can produce detectable pulmonary damage, and that dietary vitamin E alters pulmonary susceptibility to ozone exposure.},
doi = {10.1016/0013-9351(81)90161-4},
journal = {Environ. Res.; (United States)},
number = ,
volume = 24:2,
place = {United States},
year = {1981},
month = {4}
}