Fermentation of cellodextrins to ethanol using mixed-culture fermentations
The potential for enhancing ethanol production from cellodextrins by employing mixed-culture (Candida wickerhamii-Saccharomyces cerevisiae) fermentations was investigated. Initially, ethanol production was monitored in fermentation medium containing 50 g/l glucose plus 45 g/l cellobiose. Inoculum levels and times of inoculum addition were varied. Of the conditions tested, the most rapid rates of ethanol formation occurred in fermentations in which either C. wickerhamii and S. cerevisiae were coinoculated at a ratio of 57 : 1 cell/ml or in fermentations in which a 10-fold-greater S. cerevisiae inoculum was added to a pure culture C. wickerhamii fermentation after one day incubation. These conditions were used to attempt to enhance fermentations in which cellodextrins produced by trifluoroacetic acid hydrolysis of cellulose served as the sole carbon source. Cellodextrins that were not further purified after cellulose hydrolysis contained compounds that were slightly inhibitory to C. wickerhamii. In this case the mixed-culture fermentations produced 12-45% more ethanol than a pure culture C. wickerhamii fermentation. However, if the substrate was treated with Darco G-60 charcoal, the toxic materials were apparently removed and the pure culture C. wickerhamii fermentations performed as well as the mixed-culture fermentations.
- OSTI ID:
- 6451620
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 27:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CANDIDA
PERFORMANCE
DEXTRIN
FERMENTATION
SACCHAROMYCES CEREVISIAE
ACID HYDROLYSIS
BIOSYNTHESIS
CELLOBIOSE
CULTURE MEDIA
ETHANOL
GLUCOSE
MIXTURES
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
FUNGI
HEXOSES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHAROMYCES
SOLVOLYSIS
SYNTHESIS
YEASTS
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