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Title: Expression of foreign genes in filamentous cyanobacteria

Abstract

Several advantages make cyanobacteria attractive hosts for biodegradative genes and possibly for other exogenous genes that have practical uses. The authors have obtained expression in Anabaena sp. strain PCC 7120 and Nostoc ellipsosporum of a dechlorination operon, fcbAB, from Arthrobacter globiformis, and have also developed a simple method for qualitative assessment of dechlorination by microorganisms, such as cyanobacteria, whose metabolism is dependent on the presence of chloride in the medium. Transcription of fcbAB under the control of a variety of promoters was monitored by placing luxAB (encoding luciferase) downstream from fcbAB, and by measuring light emission from luciferase. They believe that the system that they have described has value as a means to screen for factors influencing transcription of foreign genes in cyanobacteria.

Authors:
;  [1]
  1. (Michigan State Univ., East Lansing (United States))
Publication Date:
OSTI Identifier:
6442194
Alternate Identifier(s):
OSTI ID: 6442194
Report Number(s):
CONF-9308131--
Journal ID: ISSN 0022-3646; CODEN: JPYLAJ
Resource Type:
Conference
Journal Name:
Journal of Phycology; (United States)
Additional Journal Information:
Journal Volume: 29:Suppl.3; Conference: 1993 meeting of the Phycological Society of America (PSA), Ames, IA (United States), 1-5 Aug 1993; Journal ID: ISSN 0022-3646
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYANOBACTERIA; BIODEGRADATION; GENES; TRANSCRIPTION; DECHLORINATION; METABOLISM; MICROORGANISMS; CHEMICAL REACTIONS; DECOMPOSITION; DEHALOGENATION 550400* -- Genetics

Citation Formats

Kuritz, T., and Wolk, C.P. Expression of foreign genes in filamentous cyanobacteria. United States: N. p., 1993. Web.
Kuritz, T., & Wolk, C.P. Expression of foreign genes in filamentous cyanobacteria. United States.
Kuritz, T., and Wolk, C.P. Tue . "Expression of foreign genes in filamentous cyanobacteria". United States.
@article{osti_6442194,
title = {Expression of foreign genes in filamentous cyanobacteria},
author = {Kuritz, T. and Wolk, C.P.},
abstractNote = {Several advantages make cyanobacteria attractive hosts for biodegradative genes and possibly for other exogenous genes that have practical uses. The authors have obtained expression in Anabaena sp. strain PCC 7120 and Nostoc ellipsosporum of a dechlorination operon, fcbAB, from Arthrobacter globiformis, and have also developed a simple method for qualitative assessment of dechlorination by microorganisms, such as cyanobacteria, whose metabolism is dependent on the presence of chloride in the medium. Transcription of fcbAB under the control of a variety of promoters was monitored by placing luxAB (encoding luciferase) downstream from fcbAB, and by measuring light emission from luciferase. They believe that the system that they have described has value as a means to screen for factors influencing transcription of foreign genes in cyanobacteria.},
doi = {},
journal = {Journal of Phycology; (United States)},
issn = {0022-3646},
number = ,
volume = 29:Suppl.3,
place = {United States},
year = {1993},
month = {6}
}

Conference:
Other availability
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