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Title: Novel method for identifying bacterial mutants with reduced epiphytic fitness

Abstract

Leaf surfaces are a habitat for large population of diverse bacteria which, in turn, can have numerous effects on the plants on which they live. Such bacteria are considered distinct from other plant-associated bacteria and have probably acquired adaptations which allow them to tolerate the physical and chemical environments found on leaves. Some bacterial traits, such as motility or UV irradiation tolerance, are unambiguously important in epiphytic fitness. However, novel traits may condition bacterial growth or survival on leaves. The author of this study has adapted a tube ice nucleation assay to allow differentiation of mutants of an ice nucleation-active bacterial strain that colonizes leave. The study describes a technique for rapid identification of bacterial mutants with quantitatively different population sized in a natural habitat based on the measurement of ice nucleus production. 41 refs., 6 figs., 2 tabs.

Authors:
 [1]
  1. Univ. of California, Berkeley (United States)
Publication Date:
OSTI Identifier:
6438741
DOE Contract Number:  
FG03-86ER13518
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 59:5; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; BIOASSAY; HABITAT; SPECIES DIVERSITY; BIOLOGICAL ADAPTATION; ICE; LEAVES; MUTANTS; NUCLEATION; PLANTS; ULTRAVIOLET RADIATION; ELECTROMAGNETIC RADIATION; MICROORGANISMS; RADIATIONS; 550700* - Microbiology

Citation Formats

Lindow, S E. Novel method for identifying bacterial mutants with reduced epiphytic fitness. United States: N. p., 1993. Web.
Lindow, S E. Novel method for identifying bacterial mutants with reduced epiphytic fitness. United States.
Lindow, S E. 1993. "Novel method for identifying bacterial mutants with reduced epiphytic fitness". United States.
@article{osti_6438741,
title = {Novel method for identifying bacterial mutants with reduced epiphytic fitness},
author = {Lindow, S E},
abstractNote = {Leaf surfaces are a habitat for large population of diverse bacteria which, in turn, can have numerous effects on the plants on which they live. Such bacteria are considered distinct from other plant-associated bacteria and have probably acquired adaptations which allow them to tolerate the physical and chemical environments found on leaves. Some bacterial traits, such as motility or UV irradiation tolerance, are unambiguously important in epiphytic fitness. However, novel traits may condition bacterial growth or survival on leaves. The author of this study has adapted a tube ice nucleation assay to allow differentiation of mutants of an ice nucleation-active bacterial strain that colonizes leave. The study describes a technique for rapid identification of bacterial mutants with quantitatively different population sized in a natural habitat based on the measurement of ice nucleus production. 41 refs., 6 figs., 2 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6438741}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 59:5,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1993},
month = {Sat May 01 00:00:00 EDT 1993}
}