A novel tumor necrosis factor-responsive transcription factor which recognizes a regulatory element in hemopoietic growth factor genes

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.6.2950· OSTI ID:6422532

Shannon, M F; Pell, L M; Kuczek, E S; Occhiodoro, F S; Dunn, S M; Vadas, M A ^[1]; Lenardo, M J ^[2]

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1. Div. of Human Immunology, Institute of Medical and Veterinary Science, Frame Road, Adelaide 5001 (AU)
2. Lab. of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD (US)

A conserved DNA sequence element, termed cytokine 1 (CK-1), is found in the promoter regions of many hemopoietic growth factor (HGF) genes. Mutational analyses and modification interference experiments show that this sequence specifically binds a nuclear transcription factor, NF-GMa, which is a protein with a molecular mass of 43 kilodaltons. It interacts with different affinities with the CK-1-like sequence from a number of HGF genes, including granulocyte macrophage colony-stimulating factor (GM-CSF), granulocyte (G)-CSF, interleukin 3 (IL-3), and IL-5. The authors show that the level of NF-GMa binding is induced in embryonic fibroblasts by tumor necrosis factor {alpha} (TNF-{alpha}) treatment and that the CK-1 sequence from the G-CSF gene is a TNF-{alpha}-responsive enhancer in these cells.

• OSTI ID: 6422532
• Journal Information: Molecular and Cellular Biology; (USA), Vol. 10:6; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
GENES
HEMATOPOIETIC SYSTEM
GENE REGULATION
TUMOR CELLS
NECROSIS
ANTIGEN-ANTIBODY REACTIONS
DNA SEQUENCING
EXPERIMENTAL DATA
GENE REPRESSORS
TRANSCRIPTION FACTORS
ANIMAL CELLS
BODY
DATA
INFORMATION
MITOGENS
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry