Two prohormones for gastrin-releasing peptide are encoded by two mRNAs differing by 19 nucleotides

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.1.19· OSTI ID:6417167

Spindel, E R; Zilberberg, M D; Habener, J F; Chin, W W

In studies on the molecular biology of human gastrin-releasing peptide (GRP), the authors have discovered an example of a change in translational reading frame apparently produced through alternative RNA splicing. Complementary DNAs prepared from a pulmonary carcinoid tumor rich in GRP immunoreactivity had one of two different-sized internal DNA fragments after digestion with the restriction enzyme Pvu II. Nucleotide sequences of the two DNA fragments were identical except for 19 additional nucleotides present in the larger fragment. The region of the mRNA containing the 19 nucleotides corresponded to the carboxyl-terminal region of the human GRP precursor. The resulting shift in reading frame causes a difference of 10 amino acids in size and an overall sequence difference of 27 amino acids between the two GRP prohormones so formed. The change in reading frame described here is unusual in eukaryotes and is yet another mechanism to produce diversity in the generation of biological peptides.

• Research Organization: Harvard Medical School, Boston, MA (United States)
• OSTI ID: 6417167
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
MOLECULAR BIOLOGY
PEPTIDES
BIOCHEMISTRY
CARCINOMAS
DNA SEQUENCING
GASTRIN
HORMONES
HYBRIDIZATION
ION EXCHANGE CHROMATOGRAPHY
LUNGS
MAN
RECOMBINANT DNA
ANIMALS
BODY
CHEMISTRY
CHROMATOGRAPHY
DISEASES
DNA
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
POLYPEPTIDES
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RNA
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry