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Title: New scissors for cutting chromosomes

Abstract

Restriction enzymes have been the workhorses of genetic engineering for nearly 20 years now. These enzymes which cut DNA at very specific sites, have made possible the astounding progress in gene cloning and sequencing. But indispensable as they are, these scissors have their limitations. Even the best among them, the rare cutters, chop big chunks of DNA into too many pieces for easy handling. The problem is becoming particular acute now that researchers are gearing up to map and sequence the very large genomes of humans and other complex organisms. Researchers at the University of Wisconsin have come up with a simple solution to this long-standing problem in genetic engineering. They have created a new type of scissors that will cut a huge piece of DNA exactly where they want and no place else. Their technique promises to become a new tool for dissecting large genomes.

Authors:
Publication Date:
OSTI Identifier:
6414531
Resource Type:
Journal Article
Journal Name:
Science (Washington, D.C.); (USA)
Additional Journal Information:
Journal Volume: 249:4965; Journal ID: ISSN 0036-8075
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMOSOMES; CLEAVAGE; GENETIC ENGINEERING; RESEARCH PROGRAMS; DNA; DNA SEQUENCING; DNA-CLONING; ENZYMES; CLONING; CRYSTAL STRUCTURE; DNA HYBRIDIZATION; HYBRIDIZATION; MICROSTRUCTURE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Roberts, L. New scissors for cutting chromosomes. United States: N. p., 1990. Web. doi:10.1126/science.2164709.
Roberts, L. New scissors for cutting chromosomes. United States. https://doi.org/10.1126/science.2164709
Roberts, L. 1990. "New scissors for cutting chromosomes". United States. https://doi.org/10.1126/science.2164709.
@article{osti_6414531,
title = {New scissors for cutting chromosomes},
author = {Roberts, L},
abstractNote = {Restriction enzymes have been the workhorses of genetic engineering for nearly 20 years now. These enzymes which cut DNA at very specific sites, have made possible the astounding progress in gene cloning and sequencing. But indispensable as they are, these scissors have their limitations. Even the best among them, the rare cutters, chop big chunks of DNA into too many pieces for easy handling. The problem is becoming particular acute now that researchers are gearing up to map and sequence the very large genomes of humans and other complex organisms. Researchers at the University of Wisconsin have come up with a simple solution to this long-standing problem in genetic engineering. They have created a new type of scissors that will cut a huge piece of DNA exactly where they want and no place else. Their technique promises to become a new tool for dissecting large genomes.},
doi = {10.1126/science.2164709},
url = {https://www.osti.gov/biblio/6414531}, journal = {Science (Washington, D.C.); (USA)},
issn = {0036-8075},
number = ,
volume = 249:4965,
place = {United States},
year = {Fri Jul 13 00:00:00 EDT 1990},
month = {Fri Jul 13 00:00:00 EDT 1990}
}