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Title: Regulation of renal Na -K-ATPase in the rat: role of increased potassium transport

Abstract

The purpose of this study was to characterize the alterations in collecting tubule Na -K -ATPase activity produced by sustained increments in dietary potassium in the rat and to evaluate the role of aldosterone in their generation. In adrenal-intact animals, feeding a high-potassium diet or administration of a high physiological dose of aldosterone, which simulates the delivery rate of this hormone during potassium loading, caused marked increments in Na -K -ATPase activity in the cortical collecting tubule (CCT) but had no effect on the enzyme in the inner stripe of the medullary collecting tubule (MCT). A significant increase in enzyme activity was also observed after smaller dietary potassium increments and after 4 days of dietary potassium load. In adrenalectomized rats provided with physiological replacement doses of corticosterone and aldosterone, Na -K -ATPase activity in both CCT and MCT was similar to that of adrenal-intact controls but remained unchanged after 7 days on the potassium-enriched (10-fold) diet. In contrast, adrenalectomized animals receiving the high physiological dose of aldosterone displayed an increase in Na -K -ATPase activity of CCT comparable with that of adrenal-intact animals, whereas the enzyme activity in the MCT was unaffected. In conclusion, 1) following chronic potassium loading Namore » -K -ATPase activity increases significantly in the CCT with no change in its activity in the inner stripe of the MCT; 2) this increase in enzyme activity occurs in a time-dependent fashion and in proportion to the potassium load; and 3) the stimulation of Na -K -ATPase activity in adrenal-replaced rats is facilitated by augmented levels of aldosterone, such as those actually observed in adrenal-intact rats subjected to chronic potassium loading.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Chicago Pritzker School of Medicine, IL
OSTI Identifier:
6411794
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 251:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALDOSTERONE; RADIOIMMUNOASSAY; ATP; TRACER TECHNIQUES; ATP-ASE; BIOCHEMISTRY; POTASSIUM COMPOUNDS; MEMBRANE TRANSPORT; ENZYME ACTIVITY; KIDNEYS; PHOSPHORUS 32; RATS; SODIUM COMPOUNDS; TUBULES; ACID ANHYDRASES; ADRENAL HORMONES; ALDEHYDES; ALKALI METAL COMPOUNDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CHEMISTRY; CORTICOSTEROIDS; DAYS LIVING RADIOISOTOPES; ENZYMES; HORMONES; HYDROLASES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; LIGHT NUCLEI; MAMMALS; MINERALOCORTICOIDS; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHOHYDROLASES; PHOSPHORUS ISOTOPES; PREGNANES; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; RODENTS; STEROID HORMONES; STEROIDS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Mujais, S K, Chekal, M A, Hayslett, J P, and Katz, A I. Regulation of renal Na -K-ATPase in the rat: role of increased potassium transport. United States: N. p., 1986. Web.
Mujais, S K, Chekal, M A, Hayslett, J P, & Katz, A I. Regulation of renal Na -K-ATPase in the rat: role of increased potassium transport. United States.
Mujais, S K, Chekal, M A, Hayslett, J P, and Katz, A I. 1986. "Regulation of renal Na -K-ATPase in the rat: role of increased potassium transport". United States.
@article{osti_6411794,
title = {Regulation of renal Na -K-ATPase in the rat: role of increased potassium transport},
author = {Mujais, S K and Chekal, M A and Hayslett, J P and Katz, A I},
abstractNote = {The purpose of this study was to characterize the alterations in collecting tubule Na -K -ATPase activity produced by sustained increments in dietary potassium in the rat and to evaluate the role of aldosterone in their generation. In adrenal-intact animals, feeding a high-potassium diet or administration of a high physiological dose of aldosterone, which simulates the delivery rate of this hormone during potassium loading, caused marked increments in Na -K -ATPase activity in the cortical collecting tubule (CCT) but had no effect on the enzyme in the inner stripe of the medullary collecting tubule (MCT). A significant increase in enzyme activity was also observed after smaller dietary potassium increments and after 4 days of dietary potassium load. In adrenalectomized rats provided with physiological replacement doses of corticosterone and aldosterone, Na -K -ATPase activity in both CCT and MCT was similar to that of adrenal-intact controls but remained unchanged after 7 days on the potassium-enriched (10-fold) diet. In contrast, adrenalectomized animals receiving the high physiological dose of aldosterone displayed an increase in Na -K -ATPase activity of CCT comparable with that of adrenal-intact animals, whereas the enzyme activity in the MCT was unaffected. In conclusion, 1) following chronic potassium loading Na -K -ATPase activity increases significantly in the CCT with no change in its activity in the inner stripe of the MCT; 2) this increase in enzyme activity occurs in a time-dependent fashion and in proportion to the potassium load; and 3) the stimulation of Na -K -ATPase activity in adrenal-replaced rats is facilitated by augmented levels of aldosterone, such as those actually observed in adrenal-intact rats subjected to chronic potassium loading.},
doi = {},
url = {https://www.osti.gov/biblio/6411794}, journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 251:2,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 1986},
month = {Fri Aug 01 00:00:00 EDT 1986}
}