In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.8877151· OSTI ID:6409338

Griffiths, W C; Camara, P D; Saritelli, A; Gentile, J

The metabolism and toxicity of the ubiquitous plasticizer, bis-(2-ethylhexyl) phthalate (DEHP), and its principal metabolite, mono-(2-ethylhexyl) phthalate (MEHP), have been extensively investigated. In an attempt to understand their disposition in man, the authors studied the in vitro serum protein-binding characteristics of these compounds, using ultracentrifugation and agarose gel electrophoresis. The association of DEHP and lipoproteins was shown to be highly dependent upon, and proportional to, the lipid concentration of the serum. It appears that more than half of the serum DEHP is bound to proteins with density greater than 1.21 g/mL when the concentration of cholesterol is below 300 mg/dL or the cholesterol and triglyceride total concentration is less than 600 mg/dL. As the cholesterol and triglyceride concentrations increase, the percent DEHP bound to VLDL, IDL, and LDL increases. MEHP is bound principally to nonlipoprotein constituents in the serum, and this binding distribution is unaffected by lipid concentration. The percent binding of DEHP and MEHP to individual proteins was also found to be unaffected by their concentrations in serum. These data indicate that the protein-binding characteristics of these compounds, in vitro, is somewhat more complex than previously reported.

• Research Organization: Brown Univ., Providence, RI (United States)
• OSTI ID: 6409338
• Journal Information: Environ. Health Perspect.; (United States), Vol. 77
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ELECTROPHORESIS
PHTHALATES
METABOLITES
PROTEINS
BIOCHEMISTRY
CHOLESTEROL
LIQUID COLUMN CHROMATOGRAPHY
SCINTILLATION COUNTING
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
CARBOXYLIC ACID SALTS
CENTRIFUGATION
CHEMISTRY
CHROMATOGRAPHY
COUNTING TECHNIQUES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
STEROIDS
STEROLS
550201* - Biochemistry- Tracer Techniques