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Title: In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate

Abstract

The metabolism and toxicity of the ubiquitous plasticizer, bis-(2-ethylhexyl) phthalate (DEHP), and its principal metabolite, mono-(2-ethylhexyl) phthalate (MEHP), have been extensively investigated. In an attempt to understand their disposition in man, the authors studied the in vitro serum protein-binding characteristics of these compounds, using ultracentrifugation and agarose gel electrophoresis. The association of DEHP and lipoproteins was shown to be highly dependent upon, and proportional to, the lipid concentration of the serum. It appears that more than half of the serum DEHP is bound to proteins with density greater than 1.21 g/mL when the concentration of cholesterol is below 300 mg/dL or the cholesterol and triglyceride total concentration is less than 600 mg/dL. As the cholesterol and triglyceride concentrations increase, the percent DEHP bound to VLDL, IDL, and LDL increases. MEHP is bound principally to nonlipoprotein constituents in the serum, and this binding distribution is unaffected by lipid concentration. The percent binding of DEHP and MEHP to individual proteins was also found to be unaffected by their concentrations in serum. These data indicate that the protein-binding characteristics of these compounds, in vitro, is somewhat more complex than previously reported.

Authors:
; ; ;
Publication Date:
Research Org.:
Brown Univ., Providence, RI (USA)
OSTI Identifier:
6409338
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environ. Health Perspect.; (United States); Journal Volume: 77
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; ELECTROPHORESIS; PHTHALATES; METABOLITES; PROTEINS; BIOCHEMISTRY; CHOLESTEROL; LIQUID COLUMN CHROMATOGRAPHY; SCINTILLATION COUNTING; THIN-LAYER CHROMATOGRAPHY; TRITIUM COMPOUNDS; ULTRACENTRIFUGATION; CARBOXYLIC ACID SALTS; CENTRIFUGATION; CHEMISTRY; CHROMATOGRAPHY; COUNTING TECHNIQUES; HYDROXY COMPOUNDS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; SEPARATION PROCESSES; STEROIDS; STEROLS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Griffiths, W.C., Camara, P.D., Saritelli, A., and Gentile, J. In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate. United States: N. p., 1988. Web. doi:10.1289/ehp.8877151.
Griffiths, W.C., Camara, P.D., Saritelli, A., & Gentile, J. In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate. United States. doi:10.1289/ehp.8877151.
Griffiths, W.C., Camara, P.D., Saritelli, A., and Gentile, J. Fri . "In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate". United States. doi:10.1289/ehp.8877151.
@article{osti_6409338,
title = {In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate},
author = {Griffiths, W.C. and Camara, P.D. and Saritelli, A. and Gentile, J.},
abstractNote = {The metabolism and toxicity of the ubiquitous plasticizer, bis-(2-ethylhexyl) phthalate (DEHP), and its principal metabolite, mono-(2-ethylhexyl) phthalate (MEHP), have been extensively investigated. In an attempt to understand their disposition in man, the authors studied the in vitro serum protein-binding characteristics of these compounds, using ultracentrifugation and agarose gel electrophoresis. The association of DEHP and lipoproteins was shown to be highly dependent upon, and proportional to, the lipid concentration of the serum. It appears that more than half of the serum DEHP is bound to proteins with density greater than 1.21 g/mL when the concentration of cholesterol is below 300 mg/dL or the cholesterol and triglyceride total concentration is less than 600 mg/dL. As the cholesterol and triglyceride concentrations increase, the percent DEHP bound to VLDL, IDL, and LDL increases. MEHP is bound principally to nonlipoprotein constituents in the serum, and this binding distribution is unaffected by lipid concentration. The percent binding of DEHP and MEHP to individual proteins was also found to be unaffected by their concentrations in serum. These data indicate that the protein-binding characteristics of these compounds, in vitro, is somewhat more complex than previously reported.},
doi = {10.1289/ehp.8877151},
journal = {Environ. Health Perspect.; (United States)},
number = ,
volume = 77,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1988},
month = {Fri Apr 01 00:00:00 EST 1988}
}