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Title: Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei. Progress report, January 31-December 15, 1985

Abstract

This work addresses the metabolism of fatty acids and the energetics of growth of the anaerobic, syntrophic, fatty acid-degrading bacterium, Syntrophomonas wolfei. S. wolfei degrades C/sub 4/ to C/sub 8/ straight chain fatty acids to acetate and H/sub 2/ or acetate, propionate and H/sub 2/; isoheptanoate is degraded to isovalerate, acetate, and H/sub 2/. S. wolfei can not use any common bacterial energy source that will allow it to grow in pure culture. A significant breakthrough in the cultivation of S. wolfei was achieved. Long term (3 months) incubation of S. wolfei cocultures in medium with crotonate selects for a population of S. wolfei cells that can use this compound. These cultures contain large numbers of S. wolfei cells and very few cells of the methanogen. Pure cultures of S. wolfei do not use butyrate. However, when pure cultures of S. wolfei are incubated in the presence of H/sub 2/-using bacteria, butyrate is degraded to acetate and H/sub 2/. These data show that the cells present in the pure cultures are in fact S. wolfei. Growth of S. wolfei with crotonate is faster and much higher cell densities are obtained. Thus, large amounts of cell material will be available formore » biochemical studies. 3 refs.« less

Authors:
Publication Date:
Research Org.:
Oklahoma Univ., Norman (USA)
OSTI Identifier:
6407976
Report Number(s):
DOE/ER/13053-4
ON: DE86004532
DOE Contract Number:
AS05-83ER13053
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; METHANOGENIC BACTERIA; CULTIVATION TECHNIQUES; MONOCARBOXYLIC ACIDS; METABOLISM; ACETIC ACID; ANAEROBIC CONDITIONS; BIOLOGICAL ADAPTATION; BUTYRIC ACID; CROTONIC ACID; EXCRETION; GAS CHROMATOGRAPHY; SYMBIOSIS; BACTERIA; CARBOXYLIC ACIDS; CHROMATOGRAPHY; CLEARANCE; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; SEPARATION PROCESSES; 090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989); 550700 - Microbiology; 550500 - Metabolism

Citation Formats

McInerney, M.J. Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei. Progress report, January 31-December 15, 1985. United States: N. p., 1985. Web.
McInerney, M.J. Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei. Progress report, January 31-December 15, 1985. United States.
McInerney, M.J. Tue . "Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei. Progress report, January 31-December 15, 1985". United States. doi:.
@article{osti_6407976,
title = {Energetics of end product excretion in anaerobic bacteria and the metabolism of fatty acids by Syntrophomonas wolfei. Progress report, January 31-December 15, 1985},
author = {McInerney, M.J.},
abstractNote = {This work addresses the metabolism of fatty acids and the energetics of growth of the anaerobic, syntrophic, fatty acid-degrading bacterium, Syntrophomonas wolfei. S. wolfei degrades C/sub 4/ to C/sub 8/ straight chain fatty acids to acetate and H/sub 2/ or acetate, propionate and H/sub 2/; isoheptanoate is degraded to isovalerate, acetate, and H/sub 2/. S. wolfei can not use any common bacterial energy source that will allow it to grow in pure culture. A significant breakthrough in the cultivation of S. wolfei was achieved. Long term (3 months) incubation of S. wolfei cocultures in medium with crotonate selects for a population of S. wolfei cells that can use this compound. These cultures contain large numbers of S. wolfei cells and very few cells of the methanogen. Pure cultures of S. wolfei do not use butyrate. However, when pure cultures of S. wolfei are incubated in the presence of H/sub 2/-using bacteria, butyrate is degraded to acetate and H/sub 2/. These data show that the cells present in the pure cultures are in fact S. wolfei. Growth of S. wolfei with crotonate is faster and much higher cell densities are obtained. Thus, large amounts of cell material will be available for biochemical studies. 3 refs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1985},
month = {Tue Jan 01 00:00:00 EST 1985}
}

Technical Report:
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