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Title: Metabolic effects of microwave radiation and convection heating on human mononuclear leukocytes. Final report, January 1985-May 1986

Abstract

Investigated here were the effects of microwave (MW) radiation (2450-MHz, continuous-wave, mean specific absorption rate of 103.5 + or - 4.2 W/kg) and convention heating on the nonphosphorylating oxidative metabolism of human peripheral mononuclear leukocytes (96% lymphocytes, 4% monocytes) at 37 C. Metabolic activity, determined by chemiluminescence (CL) of cells challenged with luminol (5-aminO-2, 3-dihydro-1, 4-phthalazinedione) linked to bovine serum albumin, was detected with a brightness photomer. A significant stimulation after after MW exposure (p < 0.005) over total CL of matched 37 C-incubator controls was observed. A similar degree of stimulation, compared to incubator controls, was also detected after sham treatment. No significant difference existed between changes in total CL or stimulation indices of the MW and sham-exposed groups. Exposure to MW radiation, under normothermic (37 + or - 0.03 C) conditions, appears to have no effect on the oxidative metabolic activity of human peripheral mononuclear leukocytes. However, the significant differences between MW or sham-exposed cells and their respective incubator controls occurred because the temperature of the incubator did not exceed 35.9 C, and 39 minutes were required for the temperature to rise from 22 to 35.9 C. Slow heating of incubator controls must be accounted for in thermalmore » and redio-frequency radiation studies in vitro.« less

Authors:
; ;
Publication Date:
Research Org.:
School of Aerospace Medicine, Brooks AFB, TX (USA)
OSTI Identifier:
6406772
Alternate Identifier(s):
OSTI ID: 6406772
Report Number(s):
AD-A-179275/3/XAB; USAFSAM-JA-86-43
Resource Type:
Technical Report
Resource Relation:
Other Information: Pub. in Physiological Chemistry and Physics and Medical NMR, Vol. 18, 181-187(1986)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEUKOCYTES; BIOLOGICAL RADIATION EFFECTS; CHEMILUMINESCENCE; CONTROL; HYDRAZIDES; HYPERTHERMIA; INCUBATION; INDEXES; METABOLISM; MICROWAVE RADIATION; OXIDATION; RADIOTHERAPY; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; BODY TEMPERATURE; CHEMICAL REACTIONS; DOCUMENT TYPES; ELECTROMAGNETIC RADIATION; LUMINESCENCE; MATERIALS; MEDICINE; NUCLEAR MEDICINE; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RADIOLOGY; THERAPY 560400* -- Other Environmental Pollutant Effects

Citation Formats

Kiel, J.L., Wong, L.S., and Erwin, D.N. Metabolic effects of microwave radiation and convection heating on human mononuclear leukocytes. Final report, January 1985-May 1986. United States: N. p., 1986. Web.
Kiel, J.L., Wong, L.S., & Erwin, D.N. Metabolic effects of microwave radiation and convection heating on human mononuclear leukocytes. Final report, January 1985-May 1986. United States.
Kiel, J.L., Wong, L.S., and Erwin, D.N. Wed . "Metabolic effects of microwave radiation and convection heating on human mononuclear leukocytes. Final report, January 1985-May 1986". United States. doi:.
@article{osti_6406772,
title = {Metabolic effects of microwave radiation and convection heating on human mononuclear leukocytes. Final report, January 1985-May 1986},
author = {Kiel, J.L. and Wong, L.S. and Erwin, D.N.},
abstractNote = {Investigated here were the effects of microwave (MW) radiation (2450-MHz, continuous-wave, mean specific absorption rate of 103.5 + or - 4.2 W/kg) and convention heating on the nonphosphorylating oxidative metabolism of human peripheral mononuclear leukocytes (96% lymphocytes, 4% monocytes) at 37 C. Metabolic activity, determined by chemiluminescence (CL) of cells challenged with luminol (5-aminO-2, 3-dihydro-1, 4-phthalazinedione) linked to bovine serum albumin, was detected with a brightness photomer. A significant stimulation after after MW exposure (p < 0.005) over total CL of matched 37 C-incubator controls was observed. A similar degree of stimulation, compared to incubator controls, was also detected after sham treatment. No significant difference existed between changes in total CL or stimulation indices of the MW and sham-exposed groups. Exposure to MW radiation, under normothermic (37 + or - 0.03 C) conditions, appears to have no effect on the oxidative metabolic activity of human peripheral mononuclear leukocytes. However, the significant differences between MW or sham-exposed cells and their respective incubator controls occurred because the temperature of the incubator did not exceed 35.9 C, and 39 minutes were required for the temperature to rise from 22 to 35.9 C. Slow heating of incubator controls must be accounted for in thermal and redio-frequency radiation studies in vitro.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}

Technical Report:
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  • The effects of microwave radiation (2450 MHz, continuous wave, mean specific absorption rate of 103.5 +/- 4.2 W/kg) and convection heating on the nonphosphorylating oxidative metabolism of human peripheral mononuclear leukocytes (96% lymphocytes, 4% monocytes) at 37 degrees C were investigated. Metabolic activity, determined by chemiluminescence (CL) of cells challenged with luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) linked to bovine serum albumin, was detected with a brightness photometer. A significant stimulation after microwave exposure (p less than 0.005) over total CL of matched 37 degrees C incubator controls was observed. A similar degree of stimulation compared to incubator controls was also detected after shammore » treatment. There was no significant difference between changes in total CL or stimulation indices of the microwave and sham exposed groups. It appears that exposure to microwave radiation, under normothermic (37 +/- 0.03 degrees C) conditions, has no effect on the oxidative metabolic activity of human peripheral mononuclear leukocytes. However, the significant differences between microwave or sham exposed cells and their respective incubator controls occurred because the temperature of the incubator controls did not exceed 35.9 degrees C and this temperature required 39 minutes to reach from 22 degrees C. Slow heating of incubator controls must be accounted for in thermal and radiofrequency radiation studies in vitro.« less
  • Human mononuclear leukocytes were exposed to 2450 MHz microwaves pulse-modulated at 16 Hz or at 60 Hz, at specific absorption rates up to 4 mW/ml. Such exposures produced no detectable effects on leukocyte viability, or on unstimulated or mitogen-stimulated DNA synthesis or total protein synthesis. The data provided no evidence that exposure to pulse-modulated microwaves is more likely to alter human leukocyte function than is exposure to continuous waves at equivalent energy levels.
  • This final report provides a summary of the goals and methods employed in a program of experimental studies of delayed neutrons carried out at the TRISTAN on-line mass separator facility at Brookhaven National Laboratory and of the principal results obtained. A list of reports and conference papers reporting the work and a short chronology of experimental runs are included. A final section is an evaluation of progress made during the contract and comparison to results obtained elsewhere. Acknowledgments and references are appended. 6 refs.
  • The Conservation and Renewable Energy Unit has completed a residential heating oil price and inventory monitoring program for the 1985-86 heating season. Price and inventory of No. 2 distillate fuels were monitored at the retail and wholesale levels on a monthly, weekly or bi-weekly basis from October 1, 1985 through May 1, 1986. A final survey was conducted on July 31 to show a more complete trend. The survey data was collected via telephone calls to a sample of fuel oil suppliers throughout the state. An analysis was performed.
  • Effects of 2450-MHz circularly polarized microwave irradiation on central nervous system functions were studied. Pulsed (microsecond, 500 pps) microwaves decreased high-affinity sodium-dependent choline uptake in the hippocampus and frontal cortex of the rat. The effect on hippocampal choline uptake was blocked by pretreatment with narcotic antagonists. Continuous-wave microwaves of the same power density decreased choline uptake in the frontal cortex only. Furthermore, it was found that the effects of pulsed microwaves on central cholinergic activity are classically conditionable to cues in the exposure environment. The hypothesis that some of the neurological effects of pulsed microwave irradiation are caused by itsmore » effect on the auditory system was investigated. Effects of pink noise and pulsed microwaves were compared.« less