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Title: Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives

Abstract

A system based on the metal-binding kidney protein, metallothionein, bound with a trace quantity of radioactive metal, has been shown to be capable of detecting parts-per-million (ppm) to parts-per-billion (ppb) concentrations of some heavy metals in liquid solution. The main objective of this study was to determine if this type of system has adequate sensitivity and selectivity for application in detecting a number of metallic species of concern to DOE, such as mercury, lead, and chromium. An affinity-displacement study is reported here using the heavy metal radiotracers {sup 65}Zn and {sup 109}Cd bound to metallothionein immobilized on an Affi-Gel 10 filter support. When a heavy metal solution with a greater affinity than the tracer for the protein is poured through the filter the radiotracer is displaced by a mechanism similar to ion exchange. The main objective of this study was to verify previous internal experimental parameters and results, and to determine the specific affinities of metallothionein for the metallic species of most concern to DOE.

Authors:
 [1]; ; ; ;  [2]
  1. Kansas State Univ., Manhattan, KS (United States)
  2. Sandia National Labs., Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Labs., Albuquerque, NM (United States)
Sponsoring Org.:
USDOE Office of Financial Management and Controller, Washington, DC (United States)
OSTI Identifier:
639710
Report Number(s):
SAND-97-2367
ON: DE98007354; TRN: 98:007919
DOE Contract Number:
AC04-94AL85000
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: Aug 1998
Country of Publication:
United States
Language:
English
Subject:
05 NUCLEAR FUELS; US DOE; REMEDIAL ACTION; SENSITIVITY ANALYSIS; PORTABLE EQUIPMENT; MEASURING INSTRUMENTS; NUMERICAL DATA; MERCURY; LEAD; CHROMIUM; METALLOTHIONEIN

Citation Formats

Randles, K.E., Bragg, D.J., Bodette, D.E., Lipinski, R.J., and Luera, T.F. Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives. United States: N. p., 1998. Web. doi:10.2172/639710.
Randles, K.E., Bragg, D.J., Bodette, D.E., Lipinski, R.J., & Luera, T.F. Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives. United States. doi:10.2172/639710.
Randles, K.E., Bragg, D.J., Bodette, D.E., Lipinski, R.J., and Luera, T.F. Sat . "Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives". United States. doi:10.2172/639710. https://www.osti.gov/servlets/purl/639710.
@article{osti_639710,
title = {Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives},
author = {Randles, K.E. and Bragg, D.J. and Bodette, D.E. and Lipinski, R.J. and Luera, T.F.},
abstractNote = {A system based on the metal-binding kidney protein, metallothionein, bound with a trace quantity of radioactive metal, has been shown to be capable of detecting parts-per-million (ppm) to parts-per-billion (ppb) concentrations of some heavy metals in liquid solution. The main objective of this study was to determine if this type of system has adequate sensitivity and selectivity for application in detecting a number of metallic species of concern to DOE, such as mercury, lead, and chromium. An affinity-displacement study is reported here using the heavy metal radiotracers {sup 65}Zn and {sup 109}Cd bound to metallothionein immobilized on an Affi-Gel 10 filter support. When a heavy metal solution with a greater affinity than the tracer for the protein is poured through the filter the radiotracer is displaced by a mechanism similar to ion exchange. The main objective of this study was to verify previous internal experimental parameters and results, and to determine the specific affinities of metallothionein for the metallic species of most concern to DOE.},
doi = {10.2172/639710},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Aug 01 00:00:00 EDT 1998},
month = {Sat Aug 01 00:00:00 EDT 1998}
}

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