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Title: Microbial degradation of alkyl carbazoles in Norman Wells crude oil

Abstract

Norman Wells crude oil was fractionated by sequential alumina and silicic acid column chromatography methods. The resulting nitrogen-rich fraction was analyzed by gas chromatography-mass spectrometry and showed 26 alkyl (C/sub 1/ to C/sub 5/) carbazoles to be the predominant compounds. An oil-degrading mixed bacterial culture was enriched on carbazole to enhance its ability to degrade nitrogen heterocycles. This culture was used to inoculate a series of flasks of mineral medium and Norman Wells crude oil. Residual oil was recovered from these cultures after incubation at 25/sup 0/C for various times. The nitrogen-rich fraction was analyzed by capillary gas chromatography, using a nitrogen-specific detector. Most of the C/sub 1/-, C/sub 2/-, and C/sub 3/- carbazoles and one of the C/sub 4/-isomers were degraded within 8 days. No further degradation occurred when incubation was extended to 28 days. The general order of susceptibility of the isomers to biodegradation was C/sub 1/ > C/sub 2/ > C/sub 3/ > C/sub 4/. The carbazole-enriched culture was still able to degrade n-alkanes, isoprenoids, aromatic hydrocarbons, and sulfur heterocycles in the crude soil. 26 references.

Authors:
;
Publication Date:
Research Org.:
Univ. of Alberta, Edmonton
OSTI Identifier:
6384881
Alternate Identifier(s):
OSTI ID: 6384881
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 47:4
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; CARBAZOLES; BIODEGRADATION; PETROLEUM; CHEMICAL COMPOSITION; ALKANES; AROMATICS; GAS CHROMATOGRAPHY; HETEROCYCLIC COMPOUNDS; MASS SPECTROSCOPY; SULFUR COMPOUNDS; TIME DEPENDENCE; AZAARENES; AZOLES; CHEMICAL REACTIONS; CHROMATOGRAPHY; DECOMPOSITION; ENERGY SOURCES; FOSSIL FUELS; FUELS; HYDROCARBONS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; SEPARATION PROCESSES; SPECTROSCOPY 023000* -- Petroleum-- Properties & Composition

Citation Formats

Fedorak, P.M., and Westlake, D.W.S.. Microbial degradation of alkyl carbazoles in Norman Wells crude oil. United States: N. p., 1984. Web.
Fedorak, P.M., & Westlake, D.W.S.. Microbial degradation of alkyl carbazoles in Norman Wells crude oil. United States.
Fedorak, P.M., and Westlake, D.W.S.. Sun . "Microbial degradation of alkyl carbazoles in Norman Wells crude oil". United States.
@article{osti_6384881,
title = {Microbial degradation of alkyl carbazoles in Norman Wells crude oil},
author = {Fedorak, P.M. and Westlake, D.W.S.},
abstractNote = {Norman Wells crude oil was fractionated by sequential alumina and silicic acid column chromatography methods. The resulting nitrogen-rich fraction was analyzed by gas chromatography-mass spectrometry and showed 26 alkyl (C/sub 1/ to C/sub 5/) carbazoles to be the predominant compounds. An oil-degrading mixed bacterial culture was enriched on carbazole to enhance its ability to degrade nitrogen heterocycles. This culture was used to inoculate a series of flasks of mineral medium and Norman Wells crude oil. Residual oil was recovered from these cultures after incubation at 25/sup 0/C for various times. The nitrogen-rich fraction was analyzed by capillary gas chromatography, using a nitrogen-specific detector. Most of the C/sub 1/-, C/sub 2/-, and C/sub 3/- carbazoles and one of the C/sub 4/-isomers were degraded within 8 days. No further degradation occurred when incubation was extended to 28 days. The general order of susceptibility of the isomers to biodegradation was C/sub 1/ > C/sub 2/ > C/sub 3/ > C/sub 4/. The carbazole-enriched culture was still able to degrade n-alkanes, isoprenoids, aromatic hydrocarbons, and sulfur heterocycles in the crude soil. 26 references.},
doi = {},
journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 47:4,
place = {United States},
year = {Sun Apr 01 00:00:00 EST 1984},
month = {Sun Apr 01 00:00:00 EST 1984}
}