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Title: Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia

Abstract

Fed-batch experiments were performed to determine the carbon tetrachloride (CT)-degrading ability of three denitrifying consortia cultured from sites not contaminated with CT. A mathematical model was used to quantify the rates of CT transformation by the consortia under both acetate-limiting and nitrate-limiting conditions. A rate constant for CT transformation on a cellular protein basis and the fraction of degraded CT transformed to chloroform (CF) were determined for each consortium by optimizing the model to fit the experimental data. The parameters for these experiments were statistically compared to those obtained for previous experiments with a denitrifying consortium cultured from an aquifer soil sample from the US Department of Energy Hanford site in southeastern Washington state. Results of F-test analysis indicated the rate of CT transformation and the production of CF both were functions of the limiting nutrient. Under nitrate-limited conditions, the rate constant for CT transformation for all four consortia was about 30 L/gmol/min and approximately 50% of the CT transformed was converted to CF. When acetate was the limiting nutrient, the rate constant for CT transformation was approximately 8 L/gmol/min and the CF yield decreased to about 25%. These results imply the ability to degrade CT may be inherent tomore » some denitrifying organisms, regardless of previous exposure to CT.« less

Authors:
;  [1];  [2]
  1. (Washington State Univ., Pullman, WA (United States). Center for Multiphase Environmental Research)
  2. (Battelle Pacific Northwest National Lab., Richland, WA (United States). Bioprocessing Group)
Publication Date:
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6378056
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 64:3; Journal ID: ISSN 0006-3592
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; ACETATES; AQUIFERS; BIODEGRADATION; CARBON TETRACHLORIDE; DENITRIFICATION; MATHEMATICAL MODELS; NITRATES; REMEDIAL ACTION; SOILS; CARBOXYLIC ACID SALTS; CHEMICAL REACTIONS; CHLORINATED ALIPHATIC HYDROCARBONS; DECOMPOSITION; HALOGENATED ALIPHATIC HYDROCARBONS; NITROGEN COMPOUNDS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXYGEN COMPOUNDS; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-)

Citation Formats

Sherwood, J.L., Petersen, J.N., and Skeen, R.S. Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia. United States: N. p., 1999. Web. doi:10.1002/(SICI)1097-0290(19990805)64:3<342::AID-BIT10>3.0.CO;2-4.
Sherwood, J.L., Petersen, J.N., & Skeen, R.S. Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia. United States. doi:10.1002/(SICI)1097-0290(19990805)64:3<342::AID-BIT10>3.0.CO;2-4.
Sherwood, J.L., Petersen, J.N., and Skeen, R.S. Thu . "Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia". United States. doi:10.1002/(SICI)1097-0290(19990805)64:3<342::AID-BIT10>3.0.CO;2-4.
@article{osti_6378056,
title = {Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia},
author = {Sherwood, J.L. and Petersen, J.N. and Skeen, R.S.},
abstractNote = {Fed-batch experiments were performed to determine the carbon tetrachloride (CT)-degrading ability of three denitrifying consortia cultured from sites not contaminated with CT. A mathematical model was used to quantify the rates of CT transformation by the consortia under both acetate-limiting and nitrate-limiting conditions. A rate constant for CT transformation on a cellular protein basis and the fraction of degraded CT transformed to chloroform (CF) were determined for each consortium by optimizing the model to fit the experimental data. The parameters for these experiments were statistically compared to those obtained for previous experiments with a denitrifying consortium cultured from an aquifer soil sample from the US Department of Energy Hanford site in southeastern Washington state. Results of F-test analysis indicated the rate of CT transformation and the production of CF both were functions of the limiting nutrient. Under nitrate-limited conditions, the rate constant for CT transformation for all four consortia was about 30 L/gmol/min and approximately 50% of the CT transformed was converted to CF. When acetate was the limiting nutrient, the rate constant for CT transformation was approximately 8 L/gmol/min and the CF yield decreased to about 25%. These results imply the ability to degrade CT may be inherent to some denitrifying organisms, regardless of previous exposure to CT.},
doi = {10.1002/(SICI)1097-0290(19990805)64:3<342::AID-BIT10>3.0.CO;2-4},
journal = {Biotechnology and Bioengineering},
issn = {0006-3592},
number = ,
volume = 64:3,
place = {United States},
year = {1999},
month = {8}
}