Retroviral and psuedogene insertion sites reveal the lineage of human salivary and pancreatic amylase genes from a single gene during primate evolution
- Michigan Univ., Ann Arbor, MI (USA). Dept. of Human Genetics
- Friedrich Meischer Inst., CH-4002 Basel (CH)
The authors have analyzed the junction regions of inserted elements within the human amylase gene complex. This complex contains five genes which are expressed at high levels either in the pancreas or in the parotid gland. The proximal 5{prime}-flanking regions of these genes contain two inserted elements. A {gamma}-actin pseudogene is located at a position 20 base pairs upstream of the first coding exon. All of the amylase genes contain this insert. The subsequent insertion of an endogenous retrovirus interrupted the {gamma}-actin pseudogene within its 3{prime}-untranslated region. Nucleotide sequence analysis of the inserted elements associated with each of the five human amylase genes has revealed a series of molecular events during the recent history of this gene family. The data indicate that the entire gene family was generated during primate evolution from one ancestral gene copy and that the retroviral insertion activated a cryptic promoter.
- OSTI ID:
- 6369756
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 10:6; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACTIN
GENES
AMYLASE
DNA SEQUENCING
GENE REGULATION
GENE AMPLIFICATION
GENE RECOMBINATION PROTEINS
GENE REPRESSORS
GENETIC ENGINEERING
MOLECULAR BIOLOGY
PANCREAS
RFLPS
SALIVARY GLANDS
VIRUSES
BODY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
NUCLEOPROTEINS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry