Signal transduction by the IL-2 receptor involves the activation of a tyrosine protein kinase
Interleukin-2 (IL-2) interacts with specific high-affinity membrane receptors to induce proliferation and/or differentiation of T lymphocytes. The ability of IL-2 to activate a tyrosine protein kinase in vivo was assessed by using antibodies to phosphotyrosine in conjunction with immunoblots. Stimulation of IL-2-dependent helper and cytotoxic T cell lines and primary cultures of human T cells with the lymphokine resulted in an increase of tyrosine phosphorylation of several proteins with molecular weights ranging from 38,000 to 120,000. The tyrosine phosphorylation in the various proteins increased in a concentrations fashion and reached a maximum level within 15 minutes. The concentrations of IL-2 required for inducing these phosphorylations and for stimulating ({sup 3}H)thymidine uptake were similar, indicating that the increase in tyrosine phosphorylation correlated with the ability of IL-2 to stimulate proliferation. Neither protein kinase C activation nor increased levels of intracellular cAMP or calcium could reproduce or inhibit the IL-2-induced tyrosine phosphorylations.
- Research Organization:
- Rochester Univ., NY (USA)
- OSTI ID:
- 6365750
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
CELL PROLIFERATION
LYMPHOKINES
RECEPTORS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
BIOCHEMICAL REACTION KINETICS
AMP
CALCIUM
CELL CULTURES
MAN
MOLECULAR WEIGHT
PHOSPHORYLATION
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TYROSINE
ALKALINE EARTH METALS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
ELEMENTS
ENZYMES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METALS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SOMATIC CELLS
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551001* - Physiological Systems- Tracer Techniques