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Title: Biochemical and proton NMR characterization of the isolated functional beta-subunit of coupling factor one from spinach chloroplasts

Abstract

Beta subunits have been dissociated from CF1 of spinach chloroplasts, purified by HPLC and characterized by two-dimensional electrophoresis and fluorescence emission. The solutions of isolated beta subunits are able to hydrolyze MgATP; this ATPase activity is an intrinsic property of the beta molecule. From proton NMR at 300 and 500 MHz, it is shown that the preparations are fully reproducible and that beta subunits remain monomeric with 75% aliphatic protons associated with rigid parts of the molecule. The other 25% give rise to separate resonances and belong to mobile side-chains and/or to flexible regions. The measurement of the transverse relaxation times T2 has permitted a detailed characterization of the molecular dynamics of the isolated beta subunits.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
C.E.N. Saclay, Gif Sur Yvette, France
OSTI Identifier:
6355130
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochem. Biophys. Res. Commun.; (United States); Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHLOROPLASTS; MOLECULAR STRUCTURE; NUCLEAR MAGNETIC RESONANCE; ATP; ELECTROPHORESIS; FLUORESCENCE SPECTROSCOPY; PLANTS; PROTONS; RELAXATION TIME; SPINACH; BARYONS; CELL CONSTITUENTS; ELEMENTARY PARTICLES; EMISSION SPECTROSCOPY; FERMIONS; FOOD; HADRONS; MAGNETIC RESONANCE; NUCLEONS; NUCLEOTIDES; ORGANIC COMPOUNDS; RESONANCE; SPECTROSCOPY; VEGETABLES; 550300* - Cytology

Citation Formats

Roux-Fromy, M., Neumann, J.M., Andre, F., Berger, G., Girault, G., Galmiche, J.M., and Remy, R.. Biochemical and proton NMR characterization of the isolated functional beta-subunit of coupling factor one from spinach chloroplasts. United States: N. p., 1987. Web. doi:10.1016/S0006-291X(87)80024-4.
Roux-Fromy, M., Neumann, J.M., Andre, F., Berger, G., Girault, G., Galmiche, J.M., & Remy, R.. Biochemical and proton NMR characterization of the isolated functional beta-subunit of coupling factor one from spinach chloroplasts. United States. doi:10.1016/S0006-291X(87)80024-4.
Roux-Fromy, M., Neumann, J.M., Andre, F., Berger, G., Girault, G., Galmiche, J.M., and Remy, R.. Wed . "Biochemical and proton NMR characterization of the isolated functional beta-subunit of coupling factor one from spinach chloroplasts". United States. doi:10.1016/S0006-291X(87)80024-4.
@article{osti_6355130,
title = {Biochemical and proton NMR characterization of the isolated functional beta-subunit of coupling factor one from spinach chloroplasts},
author = {Roux-Fromy, M. and Neumann, J.M. and Andre, F. and Berger, G. and Girault, G. and Galmiche, J.M. and Remy, R.},
abstractNote = {Beta subunits have been dissociated from CF1 of spinach chloroplasts, purified by HPLC and characterized by two-dimensional electrophoresis and fluorescence emission. The solutions of isolated beta subunits are able to hydrolyze MgATP; this ATPase activity is an intrinsic property of the beta molecule. From proton NMR at 300 and 500 MHz, it is shown that the preparations are fully reproducible and that beta subunits remain monomeric with 75% aliphatic protons associated with rigid parts of the molecule. The other 25% give rise to separate resonances and belong to mobile side-chains and/or to flexible regions. The measurement of the transverse relaxation times T2 has permitted a detailed characterization of the molecular dynamics of the isolated beta subunits.},
doi = {10.1016/S0006-291X(87)80024-4},
journal = {Biochem. Biophys. Res. Commun.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Wed Apr 29 00:00:00 EDT 1987},
month = {Wed Apr 29 00:00:00 EDT 1987}
}