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Title: Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung

Abstract

The influence of dietary selenium (Se) on the pulmonary biochemical response to ozone (O/sub 3/) exposure was examined. For 11 weeks, weanling female strain A/St mice were fed a test diet containing Se either at 0 ppm (-Se) or 1 ppm (+Se). Each diet contained 55 ppm vitamin E (vit E). Mice from each dietary group were exposed to 0.8 +/- 0.05 ppm (1568 +/- 98 micrograms/m3) O/sub 3/ continuously for 5 days. After O/sub 3/ exposure, they were killed along with a matched number of unexposed controls, and their lungs were analyzed for various biochemical parameters. The Se contents of lung tissue and whole blood were determined, and the levels were seven- to eightfold higher in +Se mice than in -Se mice, reflecting the Se intake of the animals. In unexposed control mice, Se deficiency caused a decline in glutathione peroxidase (GP) activity relative to +Se group. After O/sub 3/ exposure, the GP activity in the -Se group was associated with a lack of stimulation of glutathione reductase (GR) activity and the pentose phosphate cycle (PPC) as assessed by measuring glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities. In contrast, the +Se group after O/sub 3/ exposure exhibited increasesmore » in all four enzyme activities. Other parameters, e.g., lung weight, total lung protein, DNA and nonprotein sulfhydryl contents, and O/sub 2/ consumption, were not affected by dietary Se in the presence or absence of O/sub 3/ exposure. The data indicate that dietary Se alters the GP activity, which in turn influences the GR and PPC activities in the lung evidently through a reduced demand for NADPH. The level of vit E in the lung was found to be twofold higher in the -Se group than in the +Se group, suggesting a compensatory relationship between Se and vit E in the lung. With O3 exposure, both Se and vit E contents further increased in the lungs of each dietary group.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of California, Los Angeles
OSTI Identifier:
6353037
Resource Type:
Journal Article
Journal Name:
Toxicol. Appl. Pharmacol.; (United States)
Additional Journal Information:
Journal Volume: 71:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; OZONE; BIOLOGICAL EFFECTS; INHALATION; SELENIUM; VITAMIN E; ANTIOXIDANTS; BIOLOGICAL LOCALIZATION; BLOOD; CHEMICAL ANALYSIS; DIET; ENZYME ACTIVITY; LUNGS; MICE; RESPONSE MODIFYING FACTORS; ANIMALS; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; ELEMENTS; INTAKE; MAMMALS; MATERIALS; ORGANS; RESPIRATORY SYSTEM; RODENTS; SEMIMETALS; VERTEBRATES; VITAMINS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Elsayed, N M, Hacker, A D, Kuehn, K, Mustafa, M G, and Schrauzer, G N. Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung. United States: N. p., 1983. Web. doi:10.1016/0041-008X(83)90027-3.
Elsayed, N M, Hacker, A D, Kuehn, K, Mustafa, M G, & Schrauzer, G N. Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung. United States. https://doi.org/10.1016/0041-008X(83)90027-3
Elsayed, N M, Hacker, A D, Kuehn, K, Mustafa, M G, and Schrauzer, G N. Thu . "Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung". United States. https://doi.org/10.1016/0041-008X(83)90027-3.
@article{osti_6353037,
title = {Dietary antioxidants and the biochemical response to oxidant inhalation. II. Influence of dietary selenium on the biochemical effects of ozone exposure in mouse lung},
author = {Elsayed, N M and Hacker, A D and Kuehn, K and Mustafa, M G and Schrauzer, G N},
abstractNote = {The influence of dietary selenium (Se) on the pulmonary biochemical response to ozone (O/sub 3/) exposure was examined. For 11 weeks, weanling female strain A/St mice were fed a test diet containing Se either at 0 ppm (-Se) or 1 ppm (+Se). Each diet contained 55 ppm vitamin E (vit E). Mice from each dietary group were exposed to 0.8 +/- 0.05 ppm (1568 +/- 98 micrograms/m3) O/sub 3/ continuously for 5 days. After O/sub 3/ exposure, they were killed along with a matched number of unexposed controls, and their lungs were analyzed for various biochemical parameters. The Se contents of lung tissue and whole blood were determined, and the levels were seven- to eightfold higher in +Se mice than in -Se mice, reflecting the Se intake of the animals. In unexposed control mice, Se deficiency caused a decline in glutathione peroxidase (GP) activity relative to +Se group. After O/sub 3/ exposure, the GP activity in the -Se group was associated with a lack of stimulation of glutathione reductase (GR) activity and the pentose phosphate cycle (PPC) as assessed by measuring glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities. In contrast, the +Se group after O/sub 3/ exposure exhibited increases in all four enzyme activities. Other parameters, e.g., lung weight, total lung protein, DNA and nonprotein sulfhydryl contents, and O/sub 2/ consumption, were not affected by dietary Se in the presence or absence of O/sub 3/ exposure. The data indicate that dietary Se alters the GP activity, which in turn influences the GR and PPC activities in the lung evidently through a reduced demand for NADPH. The level of vit E in the lung was found to be twofold higher in the -Se group than in the +Se group, suggesting a compensatory relationship between Se and vit E in the lung. With O3 exposure, both Se and vit E contents further increased in the lungs of each dietary group.},
doi = {10.1016/0041-008X(83)90027-3},
url = {https://www.osti.gov/biblio/6353037}, journal = {Toxicol. Appl. Pharmacol.; (United States)},
number = ,
volume = 71:3,
place = {United States},
year = {1983},
month = {12}
}