Simultaneous fermentation and separation in an immobilized cell trickle bed reactor: Acetone-butanol-ethane (ABE) and ethanol fermentation

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6348668

Park, C H

A novel process employing immobilized cells and in-situ product removal was studied for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum and ethanol fermentation by Saccharomyces cerevisiae. Experimental studies of ABE fermentation in a trickle bed reactor without product separation showed that solvent production could be improved by one order of magnitude compared to conventional batch fermentation. Control of effluent pH near 4.3 and feed glucose concentrations higher than 10 g/L were the necessary conditions for cell growth and solvent production. A mathematical model using an equilibrium staged model predicted efficient separation of butanol from the fermentation broth. Activity coefficients of multicomponent system were estimated by Wilson's equation or the ASOG method. Inhibition by butanol and organic acids was incorporated into the kinetic expression. Experimental performance of simultaneous fermentation and separation in an immobilized cell trickle bed reactor showed that glucose conversion was improved as predicted by mathematical modeling and analysis. The effect of pH and temperature on ethanol fermentation by Saccharomyces cerevisiae was studied in free and immobilized cell reactors. Conditions for the highest glucose conversion, cell viability and least glycerol yield were determined.

• Research Organization: Purdue Univ., Lafayette, IN (USA)
• OSTI ID: 6348668
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ACETONE
YIELDS
BIOREACTORS
PERFORMANCE TESTING
BUTANOLS
ETHANOL
CLOSTRIDIUM
FERMENTATION
GLUCOSE
IMMOBILIZED CELLS
MATHEMATICAL MODELS
SACCHAROMYCES CEREVISIAE
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
EUMYCOTA
FUNGI
HEXOSES
HYDROXY COMPOUNDS
KETONES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
TESTING
YEASTS
090800* - Biomass Fuels- Production- (1990-)