Lack of a correlation between hyperthermic cell killing, thermotolerance, and membrane lipid fluidity

Journal Article · Sat Aug 01 00:00:00 EDT 1981 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575585· OSTI ID:6342200

Lepock, J R; Massicotte-Nolan, P; Rule, G S; Kruuv, J

Butylated hydroxytoluene (BHT) is an effective membrane lipid perturber. Uptake studies using (/sup 3/H)BHT showed that it is effectively taken up by V79 Chinese hamster lung cells. The correlation time of rotation (tau/sub c/) of the spin label 2,2-dimethyl-5-dodecyl-5-methyloxazolidine-N-oxide (2N14) was decreased 4.0 and 12.9% by addition of 0.03 and 0.07 mM BHT, respectively. This corresponds to increases in membrane fluidity produced by temperature increases of 1.8 and 6.0/sup 0/C, respectively. Neither BHT treatment sensitized the cells to hyperthermia. Also no decrease in membrane lipid fluidity, again as measured with the spin label 2N14, was found in thermotolerant V79 cells compared to control cells. Thermotolerance was induced by a 20-min exposure to 44.5/sup 0/C and the membrane fluidity measurements were made 16 hour later, when the maximum level of thermotolerance was observed. Thus no evidence was found for a correlation between membrane lipid fluidity and hyperthermic killing of V79 cells.

• Research Organization: Univ. of Waterloo, Ontario
• OSTI ID: 6342200
• Journal Information: Radiat. Res.; (United States), Vol. 87:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
CELL MEMBRANES
ELECTRON SPIN RESONANCE
FLUID FLOW
CRESOLS
BIOLOGICAL EFFECTS
SURVIVAL CURVES
TEMPERATURE DEPENDENCE
CELL KILLING
FIBROBLASTS
HAMSTERS
HYPERTHERMIA
RESPIRATORY TRACT CELLS
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AROMATICS
BODY TEMPERATURE
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
PHENOLS
RESONANCE
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550600 - Medicine