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Title: Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene

Abstract

Rapidly growing primary cultures of normal human mammary epithelial cells (HMEC) were exposed to 1 g of benzo(a)pyrene (B(a)P) per ml for two or three 24-hr periods. The B(a)P-treated populations consistently contained cells displaying a longer period of active growth in culture compared to the untreated control cells. Widespread heterogeneity in morphology and growth patterns was evidenced in these extended life (EL) cultures, with multiple sequential changes in these parameters occurring during the course of their life in culture. Two apparently immortal continuous cell lines have thus far emerged from these EL cultures. The continuous lines do not appear to be malignantly transformed as they do not cause tumor formation in nude mice and show little or no anchorage-independent growth. Nonetheless, they have acquired several properties characteristic of tumor-derived HMEC, which distinguish them from their normal progenitors. Analysis of the multiple steps occurring in the EL cultures, as well as in the emergence of the continuous cell lines, could potentially elucidate the processes occurring during human epithelial cell carcinogenesis and escape from senescence.

Authors:
;
Publication Date:
Research Org.:
Lawrence Berkely Lab., CA
OSTI Identifier:
6331630
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 82:8
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BENZOPYRENE; BIOLOGICAL EFFECTS; EPITHELIUM; ONCOGENIC TRANSFORMATIONS; CARCINOGENESIS; CELL CULTURES; CELL DIFFERENTIATION; CYTOLOGICAL TECHNIQUES; MAMMARY GLANDS; MORPHOLOGICAL CHANGES; ANIMAL TISSUES; AROMATICS; BODY; CELL TRANSFORMATIONS; CONDENSED AROMATICS; GLANDS; HYDROCARBONS; ORGANIC COMPOUNDS; ORGANS; PATHOGENESIS; TISSUES; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)

Citation Formats

Stampfer, M R, and Bartley, J C. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene. United States: N. p., 1985. Web. doi:10.1073/pnas.82.8.2394.
Stampfer, M R, & Bartley, J C. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene. United States. https://doi.org/10.1073/pnas.82.8.2394
Stampfer, M R, and Bartley, J C. 1985. "Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene". United States. https://doi.org/10.1073/pnas.82.8.2394.
@article{osti_6331630,
title = {Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene},
author = {Stampfer, M R and Bartley, J C},
abstractNote = {Rapidly growing primary cultures of normal human mammary epithelial cells (HMEC) were exposed to 1 g of benzo(a)pyrene (B(a)P) per ml for two or three 24-hr periods. The B(a)P-treated populations consistently contained cells displaying a longer period of active growth in culture compared to the untreated control cells. Widespread heterogeneity in morphology and growth patterns was evidenced in these extended life (EL) cultures, with multiple sequential changes in these parameters occurring during the course of their life in culture. Two apparently immortal continuous cell lines have thus far emerged from these EL cultures. The continuous lines do not appear to be malignantly transformed as they do not cause tumor formation in nude mice and show little or no anchorage-independent growth. Nonetheless, they have acquired several properties characteristic of tumor-derived HMEC, which distinguish them from their normal progenitors. Analysis of the multiple steps occurring in the EL cultures, as well as in the emergence of the continuous cell lines, could potentially elucidate the processes occurring during human epithelial cell carcinogenesis and escape from senescence.},
doi = {10.1073/pnas.82.8.2394},
url = {https://www.osti.gov/biblio/6331630}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 82:8,
place = {United States},
year = {Mon Apr 01 00:00:00 EST 1985},
month = {Mon Apr 01 00:00:00 EST 1985}
}