Transcriptional regulator of oxidative stress-inducible genes: Direct activation by oxidation

Journal Article · Fri Apr 13 00:00:00 EDT 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.2183352· OSTI ID:6325881

Storz, G; Tartaglia, L A; Ames, B N ^[1]

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1. Univ. of California, Berkeley (USA)

The oxyR gene positively regulates genes induced by oxidative stress in Salmonella typhimurium and Escherichia coli. Purification of the OxyR protein showed that oxidized but not reduced OxyR activates transcription of oxidative stress-inducible genes in vitro. Conversion between the two forms of OxyR is rapid and reversible. Both the oxidized and the reduced forms of the OxyR protein are capable of binding to three diverse sequences upstream of OxyR-regulated promoters, but the interactions of the two forms of OxyR with the promoter regions are different. The results suggest that direct oxidation of the OxyR protein brings about a conformational change by which OxyR transduces an oxidative stress signal to RNA polymerase.

• OSTI ID: 6325881
• Journal Information: Science (Washington, D.C.); (USA), Vol. 248:4952; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
GENE REGULATION
OXIDOREDUCTASES
GENES
SALMONELLA TYPHIMURIUM
PROMOTERS
RNA POLYMERASES
TRANSCRIPTION
BACTERIA
ENZYMES
MICROORGANISMS
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
SALMONELLA
TRANSFERASES
550200* - Biochemistry
550400 - Genetics