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Title: Development of DNA probes for Candida albicans

Abstract

An attempt was made to produce DNA probes that could be used as a rapid and efficient means of detecting candidiasis (invasive Candida infection) in immunocompromised patients. Whole DNA from Candida albicans was digested with restriction endonuclease, and the resulting fragments were randomly cloned into a plasmid vector. Several recombinant plasmids were evaluated for cross-hybridization to various other Candida species, other fungal DNAs, and to nonfungal DNAs. Cross reactions were observed between the probes and different yeasts, but none with unrelated DNAs. Some recombinants were genus-specific, and two of these were applied to the analysis of C. albicans growth curves. It became evident that, although both /sup 32/P- and biotin-labelled probes could be made quite sensitive, a possible limitation in their diagnostic potential was the poor liberation of Candida DNA from cells. Thus, better methods of treatment of clinical specimens will be required before such probes will be useful in routine diagnosis.

Authors:
;
Publication Date:
Research Org.:
Univ. of British Columbia, Vancouver (Canada)
OSTI Identifier:
6325048
Resource Type:
Journal Article
Journal Name:
Diagn. Microbiol. Infect. Dis.; (United States)
Additional Journal Information:
Journal Volume: 10:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CANDIDA; DIAGNOSIS; NUCLEIC ACIDS; RADIOASSAY; AUTORADIOGRAPHY; BIOTIN; DNA HYBRIDIZATION; DNA-CLONING; PHOSPHORUS 32; PLASMIDS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CLONING; DAYS LIVING RADIOISOTOPES; EUMYCOTA; FUNGI; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYBRIDIZATION; IMIDAZOLES; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOSPHORUS ISOTOPES; PLANTS; RADIOISOTOPES; VITAMIN B GROUP; VITAMINS; YEASTS; 550901* - Pathology- Tracer Techniques

Citation Formats

Cheung, L.L., and Hudson, J.B. Development of DNA probes for Candida albicans. United States: N. p., 1988. Web. doi:10.1016/0732-8893(88)90037-5.
Cheung, L.L., & Hudson, J.B. Development of DNA probes for Candida albicans. United States. doi:10.1016/0732-8893(88)90037-5.
Cheung, L.L., and Hudson, J.B. Fri . "Development of DNA probes for Candida albicans". United States. doi:10.1016/0732-8893(88)90037-5.
@article{osti_6325048,
title = {Development of DNA probes for Candida albicans},
author = {Cheung, L.L. and Hudson, J.B.},
abstractNote = {An attempt was made to produce DNA probes that could be used as a rapid and efficient means of detecting candidiasis (invasive Candida infection) in immunocompromised patients. Whole DNA from Candida albicans was digested with restriction endonuclease, and the resulting fragments were randomly cloned into a plasmid vector. Several recombinant plasmids were evaluated for cross-hybridization to various other Candida species, other fungal DNAs, and to nonfungal DNAs. Cross reactions were observed between the probes and different yeasts, but none with unrelated DNAs. Some recombinants were genus-specific, and two of these were applied to the analysis of C. albicans growth curves. It became evident that, although both /sup 32/P- and biotin-labelled probes could be made quite sensitive, a possible limitation in their diagnostic potential was the poor liberation of Candida DNA from cells. Thus, better methods of treatment of clinical specimens will be required before such probes will be useful in routine diagnosis.},
doi = {10.1016/0732-8893(88)90037-5},
journal = {Diagn. Microbiol. Infect. Dis.; (United States)},
number = ,
volume = 10:3,
place = {United States},
year = {1988},
month = {7}
}