REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis

Journal Article · Sun Jan 01 00:00:00 EST 1989 · J. Bacteriol.; (United States)

OSTI ID:6321999

Larimer, F W; Perry, J R; Hardigree, A A

The REV1 gene of Saccharomyces cerevisiae is required for normal induction of mutations by physical and chemical agents. We have determined the sequence of a 3,485-base-pair segment of DNA that complements the rev1-1 mutant. Gene disruption was used to confirm that this DNA contained the REV1 gene. The sequenced segment contains a single long open reading frame, which can encode a polypeptide of 985 amino acid residues. The REV1 transcript is 3.1 kilobase pairs in length. Frameshift mutations introduced into the open reading frame yielded a Rev-phenotype. A base substitution, encoding Gly-193 to Arg-193, was found in this open reading frame in rev1-1. Deletion mutants, lacking segments of the 5' region of REV1, had intermediate mutability relative to REV1 and rev1-1; a complete deletion exhibited lower mutability than rev1-1. REV1 is not an essential gene. An in-frame fusion of the 5' end of the REV1 open reading frame to the lacZ gene produced beta-galactosidase activity constitutively. The predicted REV1 protein is hydrophilic, with a predicted pI of 9.82. No homologies to RAD1, RAD2, RAD3, RAD7, or RAD10 proteins were noted. A 152-residue internal segment displayed 25% identity with UMUC protein.

• Research Organization: Oak Ridge National Lab., TN (USA)
• OSTI ID: 6321999
• Journal Information: J. Bacteriol.; (United States), Vol. 171:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
GALACTOSIDASE
ENZYME ACTIVITY
SACCHAROMYCES CEREVISIAE
GENES
XENOBIOTICS
GENETIC EFFECTS
AMINO ACID SEQUENCE
COSMIDS
HEAT
MUTATIONS
PHENOTYPE
TRANSCRIPTION
BIOLOGICAL EFFECTS
ENERGY
ENZYMES
EUMYCOTA
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MOLECULAR STRUCTURE
O-GLYCOSYL HYDROLASES
PLANTS
SACCHAROMYCES
YEASTS
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry