Hemodynamic and permeability characteristics of acute experimental necrotizing enterocolitis

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Digestive Diseases and Sciences; (USA)

DOI:https://doi.org/10.1007/BF01536416· OSTI ID:6316345

Miller, M J; Adams, J; Gu, X A; Zhang, X J; Clark, D A ^[1]

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1. Louisiana State Univ., New Orlean (USA)

We examined the local hemodynamic response of intestinal loops during acute necrotizing enterocolitis (NEC) in anesthetized rabbits. NEC was induced in ileal loops by transmural injection of a solution containing casein (10 mg/ml) and calcium gluconate (50 mg/ml) acidified to pH 4.0 with propionic or acetic acid. Control loops received casein only (pH 5.0). Mucosal damage was quantified by the blood-to-lumen movement of (51Cr)EDTA, fluid shifts into the lumen, and histology. Mean arterial pressure and loop blood flow were steady over the 3-hr period, loop fluid volume decreased, and there was no evidence of necrosis or epithelial damage. In loops receiving acidified casein and calcium gluconate, there was an immediate dramatic increase in loop blood flow that returned to baseline by 50 min. In addition, loop fluid volume was dramatically increased, necrosis was noted in the form of blunting and loss of villi, and sevenfold increase in (51Cr)EDTA permeability was evident. Administration of CV 1808 (30 mg/kg/hr), a selective adenosine2 agonist, which maintained and elevated loop blood flow throughout the 3 hr protocol, failed to alter the changes in loop fluid volume or prevent necrosis. Histamine levels in loop fluid levels were significantly elevated 20-30 min after NEC induction when compared to saline controls, indicating an early activation of mucosal defenses with this luminal insult. Thus, this model of NEC is characterized by a transient, acute hyperemia, increased intestinal permeability, and histamine release. As mucosal damage was independent of ischemia and could not be prevented by vasodilatory therapy, this model supports the clinical findings that NEC is correlated with luminal factors related to feeding and independent of cardiovascular stress.

• OSTI ID: 6316345
• Journal Information: Digestive Diseases and Sciences; (USA), Vol. 35:10; ISSN 0163-2116
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIGESTIVE SYSTEM DISEASES
PATHOGENESIS
EPITHELIUM
PERMEABILITY
ADENOSINE
BLOOD FLOW
BLOOD PRESSURE
CASEIN
CHROMIUM 51
EDTA
HISTAMINE
INTESTINES
NECROSIS
RABBITS
TRACER TECHNIQUES
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPES
RIBOSIDES
TISSUES
VERTEBRATES
550901* - Pathology- Tracer Techniques