Gas chromatography and gateway sensors for on-line-state estimation of complex fermentations (butanol-acetone fermentation)
Abstract
A fermentation system has been designed to demonstrate the use of gas chromatography (GC) for on-line monitoring of the butanol-acetone and other complex saccharolytic fermentations. Tangential flow ultrafiltration was used to sterilely and continuously obtain a cell-free filtrate from the fermentation broth for on-line GC analysis of butanol, butyrate, acetate, acetone, ethanol, and acetoin. The liquid injection system consists of a phosphoric acid contractor, a slider-type injection valve, and a heater to address the difficulties (ghosting) encountered in the analysis of carboxylic acids. The fermentor headspace gas was also analyzed by on-line GC for nitrogen and carbon dioxide, while hydrogen was measured by difference. Raw chromatographic data were analyzed by a chromatography data system. Both raw and processed data were transmitted to a VAX 11/750 computer for further processing (using the fermentation equation) and archiving. The fermentation equation, which has recently been derived and tested on completed fermentation data, was also found to be valid during transient fermentations and thus useful as a gateway sensor for calculating various fermentation parameters on-line. Such parameters include glucose concentration and gas composition, as well as a number of unobservable parameters (such as Y ATP, excess ATP, and NAD reduced by FdH/sub 2/), whichmore »
- Authors:
- Publication Date:
- Research Org.:
- Rice Univ., Houston, TX
- OSTI Identifier:
- 6316041
- Resource Type:
- Journal Article
- Journal Name:
- Biotechnol. Bioeng.; (United States)
- Additional Journal Information:
- Journal Volume: 27:8
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; ACETONE; MONITORING; BIOREACTORS; ON-LINE MEASUREMENT SYSTEMS; BUTANOLS; CARBON DIOXIDE; ETHANOL; NITROGEN; ACETATES; COMPUTER CALCULATIONS; CONTINUOUS CULTURE; DESIGN; FERMENTATION; GAS CHROMATOGRAPHY; PERFORMANCE; ULTRAFILTRATION; ALCOHOLS; BIOCONVERSION; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACID SALTS; CHALCOGENIDES; CHROMATOGRAPHY; ELEMENTS; FILTRATION; HYDROXY COMPOUNDS; KETONES; NONMETALS; ON-LINE SYSTEMS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; SEPARATION PROCESSES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
Citation Formats
McLaughlin, J K, Meyer, C L, and Papoutsakis, E T. Gas chromatography and gateway sensors for on-line-state estimation of complex fermentations (butanol-acetone fermentation). United States: N. p., 1985.
Web. doi:10.1002/bit.260270821.
McLaughlin, J K, Meyer, C L, & Papoutsakis, E T. Gas chromatography and gateway sensors for on-line-state estimation of complex fermentations (butanol-acetone fermentation). United States. https://doi.org/10.1002/bit.260270821
McLaughlin, J K, Meyer, C L, and Papoutsakis, E T. 1985.
"Gas chromatography and gateway sensors for on-line-state estimation of complex fermentations (butanol-acetone fermentation)". United States. https://doi.org/10.1002/bit.260270821.
@article{osti_6316041,
title = {Gas chromatography and gateway sensors for on-line-state estimation of complex fermentations (butanol-acetone fermentation)},
author = {McLaughlin, J K and Meyer, C L and Papoutsakis, E T},
abstractNote = {A fermentation system has been designed to demonstrate the use of gas chromatography (GC) for on-line monitoring of the butanol-acetone and other complex saccharolytic fermentations. Tangential flow ultrafiltration was used to sterilely and continuously obtain a cell-free filtrate from the fermentation broth for on-line GC analysis of butanol, butyrate, acetate, acetone, ethanol, and acetoin. The liquid injection system consists of a phosphoric acid contractor, a slider-type injection valve, and a heater to address the difficulties (ghosting) encountered in the analysis of carboxylic acids. The fermentor headspace gas was also analyzed by on-line GC for nitrogen and carbon dioxide, while hydrogen was measured by difference. Raw chromatographic data were analyzed by a chromatography data system. Both raw and processed data were transmitted to a VAX 11/750 computer for further processing (using the fermentation equation) and archiving. The fermentation equation, which has recently been derived and tested on completed fermentation data, was also found to be valid during transient fermentations and thus useful as a gateway sensor for calculating various fermentation parameters on-line. Such parameters include glucose concentration and gas composition, as well as a number of unobservable parameters (such as Y ATP, excess ATP, and NAD reduced by FdH/sub 2/), which characterize the state of the fermentation.},
doi = {10.1002/bit.260270821},
url = {https://www.osti.gov/biblio/6316041},
journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 27:8,
place = {United States},
year = {Thu Aug 01 00:00:00 EDT 1985},
month = {Thu Aug 01 00:00:00 EDT 1985}
}