Measurement of adenosine triphosphate (ATP) content in single red blood cells using the firefly bioluminescent reaction

doi:10.2172/6315338

Abstract

A unique optical instrument is described which uses the firefly bioluminscent reaction to measure adenosine triphosphate (ATP) levels in single red blood cells. The method allows chemical content level to be associated with individual cell features. The optical instrument consists of a phase contrast microscope to view cells, a pulsed argon-ion laser to rupture the cell membrane, and a photon counting system to measure the bioluminescent yield. The technique has been calibrated against a standard ATP measurement using bulk analysis methods. The ATP loss mechanism for blood cells in a controlled depletion experiment was also investigated.

Dense (aged) circulating red cells contain normal concentrations of adenosine triphosphate (ATP)

Kirkpatrick, F.H. ; Muhs, A.G. ; Kostuk, R.K. ; ... October 1979 - Blood; (United States)

The densest 0.1% to 1% of circulating red cells were separated from fresh blood, and the ATP content of a representative sample of such cells was determined. The dense (old) cells had decreased amounts of ATP relative to unfractionated cells. However, the dense cells were also smaller, and the concentration of ATP in these cells was the same as in controls. Therefore, it seems unlikely that loss of cellular ATP is a causative factor in removal of senescent red cells from the circulation.
Adenosine triphosphate (ATP) in the marine environment: a bibliography

Jones, A.T. ; Hartwig, E.O. ; Quinby-Hunt, M.S. March 1981

This bibliography lists published works on adenosine triphosphate (ATP) detected in the marine environment. Over 100 citations are listed in four categories: field measurements; macroscopic organisms; benthic populations; and papers on analytical methods.
REDUCED RED CELL 2,3-DIPHOSPHOGLYCERATE AND ADENOSINE TRIPHOSPHATE, HYPOPHOSPHATEMIA AND INCREASED HEMOGLOBIN--OXYGEN AFFINITY AFTER CARDIAC SURGERY.

Young, J.A. ; Lichtman, M.A. ; Cohen, J. January 1972
MEASUREMENT OF THE WATER EXCHANGE RATE OF BOUND WATER IN THE MANGANESE(II)-- ADENOSINE TRIPHOSPHATE COMPLEX BY $sup 17$O NUCLEAR MAGNETIC RESONANCE.

Zetter, M.S. ; Dodgen, H.W. ; Hunt, J.P. October 1972
Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report

Sylvia Daunert February 2000

During this funding period, the laboratories of Drs. Anderson and Daunert have performed a considerable amount of work toward addressing the issues associated with small volume analysis necessary for single cell studies. In that respect, their research has been focused on (1) developing new assays that can be miniaturized and are suitable for small volume and single cell analysis; (2) fabricating pL-vials that simulate the volume of single cells and setting up instrumentation capable of low-volume detection; (3) developing reproducible and reliable microinjection techniques; (4) developing methods of analysis for biomolecules in the pL-vials and employing these assays in themore »« less
DOI: 10.2172/755430

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Title: Measurement of adenosine triphosphate (ATP) content in single red blood cells using the firefly bioluminescent reaction

Abstract

Citation Formats

Dense (aged) circulating red cells contain normal concentrations of adenosine triphosphate (ATP)

Adenosine triphosphate (ATP) in the marine environment: a bibliography

REDUCED RED CELL 2,3-DIPHOSPHOGLYCERATE AND ADENOSINE TRIPHOSPHATE, HYPOPHOSPHATEMIA AND INCREASED HEMOGLOBIN--OXYGEN AFFINITY AFTER CARDIAC SURGERY.

MEASUREMENT OF THE WATER EXCHANGE RATE OF BOUND WATER IN THE MANGANESE(II)-- ADENOSINE TRIPHOSPHATE COMPLEX BY $sup 17$O NUCLEAR MAGNETIC RESONANCE.

Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report