Acrylamide exposure induces a delayed unscheduled DNA synthesis in germ cells of male mice that is correlated with the temporal pattern of adduct formation in testis DNA

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850160302· OSTI ID:6314928

Sega, G A; Generoso, E E; Brimer, P A ^[1]

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1. Oak Ridge National Laboratory, TN (USA)

A study of meiotic and postmeiotic germ-cell-stage sensitivity of male mice to induction of unscheduled DNA synthesis (UDS) by acrylamide showed that DNA repair could be detected in early spermatocytes (after the last scheduled DNA synthesis) through about mid-spermatid stages. No DNA repair could be detected in later stages. The maximum UDS response was observed 6 hr after i.p. exposure and was about 5 times greater than the response measured immediately after treatment. This is the longest delay between chemical treatment and maximum UDS response yet observed in mouse germ cells. There was a linear relationship between the UDS response and acrylamide exposure from 7.8 to 125 mg/kg. By using 14C-labeled acrylamide it was determined that the temporal pattern of adduct formation in testes DNA paralleled that of the UDS response, with maximum binding occurring 4 to 6 hr after exposure. In contrast, the temporal pattern of adduct formation in liver DNA showed maximum binding within 1 to 2 hr after exposure and was an order of magnitude greater than that found for the testis DNA.

• OSTI ID: 6314928
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Vol. 16:3; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ACRYLAMIDE
METABOLISM
DNA ADDUCTS
BIOSYNTHESIS
SPERMATOCYTES
DNA REPLICATION
CARBON 14 COMPOUNDS
DNA REPAIR
MALES
MICE
MUTATIONS
TESTES
TRACER TECHNIQUES
ADDUCTS
AMIDES
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
GERM CELLS
GONADS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RECOVERY
REPAIR
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques