Comparison of 6-thioguanine-resistant mutation and sister chromatid exchanges in Chinese hamster V79 cells with forty chemical and physical agents
The induction of sister chromatid exchanges (SCE) and mutation at the hypoxanthine-guanine phosphoribosyl transferase locus and toxicities of 40 different chemical and physical agents were examined on Chinese hamster V79 cells. These agents included mono-, di-, tri-, and polyfunctional alkylating agents, intercalators, gamma-rays, and UV light irradiation. Mutation was measured as resistance to 6-thioguanine and toxicity as loss of cell-plating efficiency. SCE were examined 29 hr after treatment. With the agents examined, a highly positive correlation existed between SCE-inducing and mutagenic potencies, when expressed as increase in the number per a unit dose over the control values. But the great difference of the ratios of mutagenic potencies versus SCE-inducing potencies among agents was observed, the maximal difference in the ratios being about 200-fold. The agents that showed the higher values of the ratio (agents producing more mutations than SCE) were bleomycin, cobalt-60 gamma-rays, all ethylating agents (N-ethyl-N-nitrosourea, N-ethyl-N'-nitro-N-nitrosoguanidine, ethyl methanesulfonate, and diethylsulfate), N-propyl-N-nitrosourea, N-butyl-N-nitrosourea, isopropyl methanesulfonate, intercalating acridine compounds (2-methoxy-6-chloro-9-(3-(ethyl-2-chloroethyl)aminopropylamino)-acridine X 2HCl and 2-methoxy-6-chloro-9-(3-(chloroethyl)-aminopropylamino)acridine 2HCl) and UV light at 254 nm.
- Research Organization:
- Japan Tobacco and Salt Public Corp., Kanagawa
- OSTI ID:
- 6304795
- Journal Information:
- Cancer Res.; (United States), Vol. 44:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACRIDINES
MUTAGENESIS
ALKYLATING AGENTS
GAMMA RADIATION
ULTRAVIOLET RADIATION
CHO CELLS
SISTER CHROMATID EXCHANGES
TOXICITY
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
RADIATIONS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)