20-kDa protein associated with the murine T-cell antigen receptor is phosphorylated in response to activation by antigen or concanavalin A

Journal Article · Mon Apr 01 00:00:00 EST 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.7.1969· OSTI ID:6301220

Samelson, L E; Harford, J; Schwartz, R H; Klausner, R D

Antigen or concanavalin A activation of a murine T-cell hybrid specific for pigeon cytochrome resulted in phosphorylation of a 20-kDa protein that was specifically coprecipitated by a monoclonal antibody binding the T-cell antigen receptor. There was no evidence for phosphorylation of the antigen receptor itself. The phosphorylation of the 20-kDa polypeptide was dependent on the concentration of antigen or lectin used to activate the T-cell hybrid and reached a maximum 40 min after the addition of antigen. The 20-kDa protein was also radioiodinated with a hydrophobic photoactivatable labeling reagent. The amount of iodinated 20-kDa protein immunoprecipitable with the anti-receptor antibody did not increase with T-cell activation, indicating that the phosphorylation occurred on a molecule that was constitutively associated with the antigen receptor. Concanavalin A also induced phosphorylation of a 20-kDa polypeptide in a second antigen-specific major histocompatibility complex-restricted T-cell hybrid. Again, the phosphorylated polypeptide was precipitated only by a monoclonal antibody specific for the antigen receptor on this hybrid. Thus, the antigen or concanavalin A-induced activation of T-cell hybrids results in the rapid phosphorylation of a 20-kDa protein that is associated with the T-cell receptor.

• Research Organization: National Institute of Health, Bethesda, MD
• OSTI ID: 6301220
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
RADIOASSAY
LYMPHOCYTES
CHEMICAL ACTIVATION
POLYPEPTIDES
PHOSPHORYLATION
CONCANAVALIN
IODINE 125
MONOCLONAL ANTIBODIES
PHOSPHORUS 32
RECEPTORS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
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