Stimulation of glucose uptake by transforming growth factor. beta. : evidence for the requirement of epidermal growth factor-receptor activation
Transforming growth factor ..beta.. (TGF-..beta..), derived from human platelets, stimulates the uptake of 2-deoxyglucose by cultured cell monolayers 2- to 4-fold. Stimulation can be detected as early as 30 min with as little as 0.1 ng of TGF-..beta.. per ml and maximal effects can be obtained at 2 hr with 1 ng of the growth factor per ml. TGF-..beta..-induced stimulation of sugar uptake is enhanced by the co-addition of platelet-derived growth factor (10 ng/ml) or epidermal growth factor (EGF, 1 ng/ml). The NR-6 variant of mouse 3T3 cells, which lack EGF receptors, is not stimulated by TGF-..beta... Antisera to EGF receptors that block /sup 125/I-labeled EGF binding also inhibit TGF-..beta.. stimulation of 2-deoxyglucose uptake, although /sup 125/I-labeled TGF-..beta.. binding remains unimpaired. In contrast, antisera to the EGF receptor, which do not block EGF binding, have no measurable effect on the TGF-..beta..-stimulated uptake of 2-deoxyglucose. The authors confirm that the receptor for TGF-..beta.. is distinct from the receptor for EGF and the authors conclude that TGF-..beta.. stimulation of 2-deoxyglucose uptake requires the co-activation of the EGF receptor kinase system.
- Research Organization:
- Vanderbilt Univ., School of Medicine, Nashville, TN
- OSTI ID:
- 6301199
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:5
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques