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Title: Ozone inhibition of photosynthesis in Chlorella sorokiniana

Abstract

Exposure of Chlorella sorokiniana (07-11-05) to ozone inhibits photosynthesis. In this study, the effects of ozone on O/sub 2/ evolution and fluorescence yields are used to characterize this inhibition. At an ozone dose of about 3 micromoles delivered to 2 x 10/sup 9/ cells, the photosynthetic rate of the cells is inhibited 50%, as indicated by a decrease in bicarbonate-stimulated O/sub 2/ evolution (control rate, 1.4 +- 0.3 x 10/sup -15/ moles per cell per minute). Normal patterns of chlorophyll fluorescence are also altered. Upon continuous exposure to ozone (3.5 x 10/sup -7/ moles O/sub 3/ per minute), three stages of change in relative fluorescence yields are observed: (a) a rise in variable yield with no corresponding change in nonvariable yield (after 1-2 minutes), which was interpreted to be a shift in the energy flow pathway; (b) a decline in variable yield with a slight rise in nonvariable yield (requiring 3-5 minutes), interpreted to be a blockage in the CO/sub 2/ fixation pathways; and (c) complete blockage of variable yield with a concurrent decline in nonvariable yield (8-10 minutes), interpreted to be a destruction of the pigment system. The timing of each stage depended upon the ozone concentration and itsmore » delivery rate to the cell suspension. These results are compared with ozone-induced decline in photosynthesis and leaf water potential changes reported for other plant systems. Evidence is also presented to suggest that ozone effects on the photosynthetic processes are attributable to ionic imbalances brought about by ozone interaction with the plasmalemma rather than a direct effect on the chloroplast. 25 references, 6 figures, 2 tables.« less

Authors:
; ;
Publication Date:
Research Org.:
Univ. of California, Riverside, CA (United States)
OSTI Identifier:
6298108
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 69:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; OZONE; BIOLOGICAL EFFECTS; BIOLOGICAL PATHWAYS; CARBON DIOXIDE FIXATION; CHLORELLA; DOSE-RESPONSE RELATIONSHIPS; INHIBITION; PHOTOSYNTHESIS; ALGAE; CHEMICAL REACTIONS; MICROORGANISMS; PHOTOCHEMICAL REACTIONS; PLANTS; SYNTHESIS; UNICELLULAR ALGAE; 560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)

Citation Formats

Heath, R L, Frederick, P E, and Chimiklis, P E. Ozone inhibition of photosynthesis in Chlorella sorokiniana. United States: N. p., 1982. Web. doi:10.1104/pp.69.1.229.
Heath, R L, Frederick, P E, & Chimiklis, P E. Ozone inhibition of photosynthesis in Chlorella sorokiniana. United States. https://doi.org/10.1104/pp.69.1.229
Heath, R L, Frederick, P E, and Chimiklis, P E. 1982. "Ozone inhibition of photosynthesis in Chlorella sorokiniana". United States. https://doi.org/10.1104/pp.69.1.229.
@article{osti_6298108,
title = {Ozone inhibition of photosynthesis in Chlorella sorokiniana},
author = {Heath, R L and Frederick, P E and Chimiklis, P E},
abstractNote = {Exposure of Chlorella sorokiniana (07-11-05) to ozone inhibits photosynthesis. In this study, the effects of ozone on O/sub 2/ evolution and fluorescence yields are used to characterize this inhibition. At an ozone dose of about 3 micromoles delivered to 2 x 10/sup 9/ cells, the photosynthetic rate of the cells is inhibited 50%, as indicated by a decrease in bicarbonate-stimulated O/sub 2/ evolution (control rate, 1.4 +- 0.3 x 10/sup -15/ moles per cell per minute). Normal patterns of chlorophyll fluorescence are also altered. Upon continuous exposure to ozone (3.5 x 10/sup -7/ moles O/sub 3/ per minute), three stages of change in relative fluorescence yields are observed: (a) a rise in variable yield with no corresponding change in nonvariable yield (after 1-2 minutes), which was interpreted to be a shift in the energy flow pathway; (b) a decline in variable yield with a slight rise in nonvariable yield (requiring 3-5 minutes), interpreted to be a blockage in the CO/sub 2/ fixation pathways; and (c) complete blockage of variable yield with a concurrent decline in nonvariable yield (8-10 minutes), interpreted to be a destruction of the pigment system. The timing of each stage depended upon the ozone concentration and its delivery rate to the cell suspension. These results are compared with ozone-induced decline in photosynthesis and leaf water potential changes reported for other plant systems. Evidence is also presented to suggest that ozone effects on the photosynthetic processes are attributable to ionic imbalances brought about by ozone interaction with the plasmalemma rather than a direct effect on the chloroplast. 25 references, 6 figures, 2 tables.},
doi = {10.1104/pp.69.1.229},
url = {https://www.osti.gov/biblio/6298108}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 69:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}