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Title: Primary structure of the Escherichia coli ribonucleotide diphosphate reductase operon

Abstract

The nucleotide sequence of the Escherichia coli K-12 DNA comprising the operon for the structural genes of the subunits of ribonucleotide diphosphate reductase has been determined. The DNA sequenced maps at 48.5 minutes on the E. coli chromosome and includes a total length of 8557 nucleotides. An open reading frame between nucleotides 3506 and 5834, encoding a 776-amino acid polypeptide chain with a molecular weight of 87,532, has been identified as the nrdA gene. An open reading frame between nucleotides 6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene. The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites. 25 references, 2 figures, 1 table.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Minnesota, St. Paul
OSTI Identifier:
6297490
DOE Contract Number:  
FG02-84ER13210
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 81
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; DNA SEQUENCING; OXIDOREDUCTASES; AMINO ACID SEQUENCE; CHROMOSOMES; ESCHERICHIA COLI; EXPERIMENTAL DATA; MOLECULAR WEIGHT; NUCLEOTIDES; STRUCTURAL CHEMICAL ANALYSIS; BACTERIA; DATA; ENZYMES; INFORMATION; MICROORGANISMS; MOLECULAR STRUCTURE; NUMERICAL DATA; ORGANIC COMPOUNDS; 550400* - Genetics

Citation Formats

Carlson, J., Fuchs, J.A., and Messing, J. Primary structure of the Escherichia coli ribonucleotide diphosphate reductase operon. United States: N. p., 1984. Web. doi:10.1073/pnas.81.14.4294.
Carlson, J., Fuchs, J.A., & Messing, J. Primary structure of the Escherichia coli ribonucleotide diphosphate reductase operon. United States. doi:10.1073/pnas.81.14.4294.
Carlson, J., Fuchs, J.A., and Messing, J. Sun . "Primary structure of the Escherichia coli ribonucleotide diphosphate reductase operon". United States. doi:10.1073/pnas.81.14.4294.
@article{osti_6297490,
title = {Primary structure of the Escherichia coli ribonucleotide diphosphate reductase operon},
author = {Carlson, J. and Fuchs, J.A. and Messing, J.},
abstractNote = {The nucleotide sequence of the Escherichia coli K-12 DNA comprising the operon for the structural genes of the subunits of ribonucleotide diphosphate reductase has been determined. The DNA sequenced maps at 48.5 minutes on the E. coli chromosome and includes a total length of 8557 nucleotides. An open reading frame between nucleotides 3506 and 5834, encoding a 776-amino acid polypeptide chain with a molecular weight of 87,532, has been identified as the nrdA gene. An open reading frame between nucleotides 6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene. The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites. 25 references, 2 figures, 1 table.},
doi = {10.1073/pnas.81.14.4294},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 81,
place = {United States},
year = {1984},
month = {7}
}