Acetylcholine receptors in the human retina

Journal Article · Fri Nov 01 00:00:00 EST 1985 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:6296842

Hutchins, J B; Hollyfield, J G

Evidence for a population of acetylcholine (ACh) receptors in the human retina is presented. The authors have used the irreversible ligand TH-propylbenzilylcholine mustard (TH-PrBCM) to label muscarinic receptors. TH- or SVI-alpha-bungarotoxin (alpha-BTx) was used to label putative nicotinic receptors. Muscarinic receptors are apparently present in the inner plexiform layer of the retina. Autoradiographic grain densities are reduced in the presence of saturating concentrations of atropine, quinuclidinyl benzilate or scopolamine; this indicates that TH-PrBCM binding is specific for a population of muscarinic receptors in the human retina. Binding sites for radiolabeled alpha-BTx are found predominantly in the inner plexiform layer of the retina. Grain densities are reduced in the presence of d-tubocurarine, indicating that alpha-BTx may bind to a pharmacologically relevant nicotinic ACh receptor. This study provides evidence for cholinergic neurotransmission in the human retina.

• Research Organization: Cullen Eye Institute, Houston, TX
• OSTI ID: 6296842
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Vol. 11
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
RECEPTORS
RADIOASSAY
RETINA
AUTORADIOGRAPHY
ACETYLCHOLINE
IODINE 125
NEUROREGULATORS
RABBITS
TRITIUM COMPOUNDS
VISION
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
BODY AREAS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EYES
FACE
HEAD
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SENSE ORGANS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics